Cell Distribution Profiles in Three-Dimensional Scaffolds with Inverted-Colloidal-Crystal Geometry: Modeling and Experimental Investigations

Authors

  • Sachin Shanbhag Dr.,

    1. Department of Chemical Engineering, University of Michigan, 3074 HH Dow Building, 2300 Hayward, Ann Arbor, MI 48109, USA, Fax: (+1) 734-763-0459
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  • Shaopeng Wang Dr.,

    1. Nomadics, Incorporated, 1024 South Innovation Way, Stillwater, Oklahoma 74074, USA
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  • Nicholas A. Kotov Prof.

    1. Department of Chemical Engineering, University of Michigan, 3074 HH Dow Building, 2300 Hayward, Ann Arbor, MI 48109, USA, Fax: (+1) 734-763-0459
    2. Department of Biomedical Engineering and Department of Materials Science & Engineering, University of Michigan, 3074 HH Dow Building, 2300 Hayward, Ann Arbor, MI 48109, USA
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Abstract

Limited ingrowth of stromal cells is observed when a three-dimensionally ordered scaffold possessing inverted-colloidal-crystal geometry is used to culture adherent cells. In this work, a computational model explaining, as well as predicting, experimental cell distributions is developed. It incorporates a modified Contois cell-growth model that includes the effects of nutrient saturation, competitive product inhibition, and cell-contact inhibition to describe the scaffold–cell system. Our results agree with the hypothesis that the rapid growth of cells on the surface of the scaffold depletes the nutrient supply to the core, resulting in the preferential growth on the exterior of the scaffold. When the cells are cultured in a scaffold subjected to a uniform velocity field, they penetrate to a greater extent into the scaffold core. Alternative seeding and culture strategies are suggested and evaluated.

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