J.K. would like to thank the U.S. Department of Energy (DOE) LDRD funds administered by the Pacific Northwest National Laboratory, DARPA/MTO under Contract DE-AC05-76 L01830, and the DOE Office of Biological and Environmental Research under the Environmental Management Science Program. T.H. would like to thank the Korean Ministry of Science and Technology for financial support through the National Creative Research Initiative Program. The research was performed in part at the W. R. Wiley Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the DOE’s Office of Biological and Environmental Research and located at the Pacific Northwest National Laboratory.
A Magnetically Separable, Highly Stable Enzyme System Based on Nanocomposites of Enzymes and Magnetic Nanoparticles Shipped in Hierarchically Ordered, Mesocellular, Mesoporous Silica†
Article first published online: 6 OCT 2005
Copyright © 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 1, Issue 12, pages 1203–1207, December 2005
How to Cite
Kim, J., Lee, J., Na, H., Kim, B., Youn, J., Kwak, J., Moon, K., Lee, E., Kim, J., Park, J., Dohnalkova, A., Park, H., Gu, M., Chang, H., Grate, Jay W. and Hyeon, T. (2005), A Magnetically Separable, Highly Stable Enzyme System Based on Nanocomposites of Enzymes and Magnetic Nanoparticles Shipped in Hierarchically Ordered, Mesocellular, Mesoporous Silica. Small, 1: 1203–1207. doi: 10.1002/smll.200500245
- Issue published online: 28 OCT 2005
- Article first published online: 6 OCT 2005
- Manuscript Received: 19 JUL 2005
- enzyme catalysis;
- magnetic nanoparticles;
- magnetic separation;
- mesoporous materials;
Multifunctional nanocomposites (M-CLEAs) of enzymes and magnetic nanoparticles (M-NPs) were fabricated in hierarchically ordered, mesocellular, mesoporous silica (HMMS; see Figure) by a simple process involving the co-adsorption of enzyme molecules and magnetic nanoparticles into HMMS followed by glutaraldehyde (GA) treatment. These nanocomposites are magnetically separable and highly stable and active. In particular, M-CLEA–lipase shows no decrease of lipase activity at all in the presence of proteases.