Gold-Conjugated Protein Nanoarrays through Block-Copolymer Lithography: From Fabrication to Biosensor Design

Authors

  • Jin-Mi Jung,

    1. Laboratory of Organic Opto-Electronic Materials, Department of Chemical & Biomolecular Engineering (BK-21), Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea, Fax: (+82) 42-869-3910
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  • Ki Young Kwon,

    1. Laboratory of Organic Opto-Electronic Materials, Department of Chemical & Biomolecular Engineering (BK-21), Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea, Fax: (+82) 42-869-3910
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  • Tai-Hwan Ha Dr.,

    1. Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong-gu, Daejeon 305-600, Korea, Fax: (+82) 42-879-8594
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  • Bong Hyun Chung Dr.,

    1. Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong-gu, Daejeon 305-600, Korea, Fax: (+82) 42-879-8594
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  • Hee-Tae Jung Prof.

    1. Laboratory of Organic Opto-Electronic Materials, Department of Chemical & Biomolecular Engineering (BK-21), Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea, Fax: (+82) 42-869-3910
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  • This work was supported by grants from the KRIBB Initiative Research Program, CUPS-ERC (KOSEF, Korea), the Protein Chip Technology Program (MOST, Korea), and MOHW (0405-MN01-0604-0007).

Abstract

original image

A hole lot of protein: A method to immobilize proteins at defined positions at the single-molecule level has been developed by using templates structured by block-copolymer lithography to generate ultrahigh-density protein nanoarrays (see image). Carbohydrate–lectin (RCA or WGA) interactions on the nanoarray surface have remarkably high selectivity and reproducibility and indicate that this approach is applicable for biosensors.

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