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Submicrometer Pore-Based Characterization and Quantification of Antibody–Virus Interactions


  • This work was supported by an NSF career award (M.M.) and by a research grant from IMRA America and AISIN USA as well as seed funds from the College of Engineering, University of Michigan. The authors thank Daniel J. Estes, James Gurnon, and James L. Van Etten for valuable discussions.


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A resistive-pulse sensor (see scheme) employs a submicrometer pore for the detection, characterization, and quantification of the binding of polyclonal antibodies to intact Paramecium bursaria chlorella virus (PBCV-1) particles. The assay is rapid, label-free, requires no immobilization or modification of the antibody or virus, detects the formation of viral aggregates, and can be performed using antibodies in complex media such as serum. The maximum number of antibodies able to bind to the virus was estimated to be 4200±450.