This work was supported by a laboratory-directed research and development program at Pacific Northwest National Laboratory (PNNL). The work was performed at the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the U.S. Department of Energy (DOE) and located at PNNL. PNNL is operated by Battelle for the DOE under Contract DE-AC05-76 L01830.
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Communication
Electroactive Silica Nanoparticles for Biological Labeling†
Article first published online: 27 JUL 2006
DOI: 10.1002/smll.200600189
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Wang, J., Liu, G. and Lin, Y. (2006), Electroactive Silica Nanoparticles for Biological Labeling. Small, 2: 1134–1138. doi: 10.1002/smll.200600189
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Publication History
- Issue published online: 29 AUG 2006
- Article first published online: 27 JUL 2006
- Manuscript Received: 21 APR 2006
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Keywords:
- biolabeling;
- biosensors;
- nanoparticles;
- proteins;
- silica
Graphical Abstract
Making biosense: Electroactive poly(guanine)-functionalized silica nanoparticles have been synthesized and used as biological labels (see scheme). An electrochemical immunobiosensor based on such labels was developed, which utilizes a mediator-generated catalytic reaction. This immunobiosensor is very sensitive for IgG detection (to a limit of 0.2 ng mL−1 or 1.3 pM), which was attributed to signal amplification from the poly(guanine)-functionalized silica nanoparticles and from the catalytic oxidation of guanine.