R.A.V and C.F.K. Shen contributed equally to this work. C.A.M. acknowledges the AFOSR, ARO, DARPA, NIH, and NSF for support of this work and R.A.L. acknowledges the NIH and HHMI for support. C.A.M. is also grateful for a NIH Director’s Pioneer Award. D.M. is grateful to the Generalitat de Catalunya for a postdoctoral grant and the Ministerio de Ciencia y Tecnologia for a Ramón y Cajal contract. R.A.L. is an Investigator of the Howard Hughes Medical Institute.
Monitoring Single-Cell Infectivity from Virus-Particle Nanoarrays Fabricated by Parallel Dip-Pen Nanolithography†
Article first published online: 13 AUG 2007
Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 3, Issue 9, pages 1482–1485, September 3, 2007
How to Cite
Vega, Rafael A., Shen, C. K.-F., Maspoch, D., Robach, Jessica G., Lamb, Robert A. and Mirkin, Chad A. (2007), Monitoring Single-Cell Infectivity from Virus-Particle Nanoarrays Fabricated by Parallel Dip-Pen Nanolithography. Small, 3: 1482–1485. doi: 10.1002/smll.200700244
- Issue published online: 4 SEP 2007
- Article first published online: 13 AUG 2007
- Manuscript Received: 7 APR 2007
- cell infection;
- dip-pen nanolithography;
Sting like a bee: Nanoarrays of infectious virus particles, encoded with EGFP, are patterned by dip-pen nanolithography and exposed to a solution of cells. Upon infection, infected cells produce the EGFP protein, generating a green fluorescence signal that allows one to monitor the cellular infection process in real time (as seen in the optical image). These results suggest that antibody-immobilized virus particles retain their biological activity. Scale bar: 35μm.