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Monitoring Single-Cell Infectivity from Virus-Particle Nanoarrays Fabricated by Parallel Dip-Pen Nanolithography

Authors

  • Rafael A. Vega,

    1. Department of Chemistry, Department of Materials Science and Engineering, Department of Medicine, and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA, Fax: (+1) 847-467-5123
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  • Clifton K.-F. Shen Dr.,

    1. Department of Chemistry, Department of Materials Science and Engineering, Department of Medicine, and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA, Fax: (+1) 847-467-5123
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  • Daniel Maspoch Dr.,

    1. Department of Chemistry, Department of Materials Science and Engineering, Department of Medicine, and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA, Fax: (+1) 847-467-5123
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  • Jessica G. Robach,

    1. Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208-3500, USA
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  • Robert A. Lamb Prof.,

    1. Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208-3500, USA
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  • Chad A. Mirkin Prof.

    1. Department of Chemistry, Department of Materials Science and Engineering, Department of Medicine, and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA, Fax: (+1) 847-467-5123
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  • R.A.V and C.F.K. Shen contributed equally to this work. C.A.M. acknowledges the AFOSR, ARO, DARPA, NIH, and NSF for support of this work and R.A.L. acknowledges the NIH and HHMI for support. C.A.M. is also grateful for a NIH Director’s Pioneer Award. D.M. is grateful to the Generalitat de Catalunya for a postdoctoral grant and the Ministerio de Ciencia y Tecnologia for a Ramón y Cajal contract. R.A.L. is an Investigator of the Howard Hughes Medical Institute.

Abstract

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Sting like a bee: Nanoarrays of infectious virus particles, encoded with EGFP, are patterned by dip-pen nanolithography and exposed to a solution of cells. Upon infection, infected cells produce the EGFP protein, generating a green fluorescence signal that allows one to monitor the cellular infection process in real time (as seen in the optical image). These results suggest that antibody-immobilized virus particles retain their biological activity. Scale bar: 35μm.

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