Dual-Color STED Microscopy at 30-nm Focal-Plane Resolution


  • We thank Gael Moneron for help with the operation of the laser system as well as Andreas Schönle and Jan Keller for valuable discussions. L.M. and D.W. contributed equally to this work.

  • Dedicated to the memory of Prof. Osamu Nakamura (1962–2004) of Osaka University, whose early interests in STED microscopy were sadly disrupted.


original image

Stimulated emission depletion (STED) fluorescence microscopy images protein distribution in cells in two-color channels at a resolution of ≈30 nm in the focal plane (see image), corresponding to a seven-to eightfold improvement over confocal microscopy resolution.