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Keywords:

  • fluorescence spectroscopy;
  • imaging;
  • optics;
  • STED
Thumbnail image of graphical abstract

Stimulated emission depletion (STED) fluorescence microscopy images protein distribution in cells in two-color channels at a resolution of ≈30 nm in the focal plane (see image), corresponding to a seven-to eightfold improvement over confocal microscopy resolution.