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Assessing Methods for Blood Cell Cytotoxic Responses to Inorganic Nanoparticles and Nanoparticle Aggregates

  1. Belén Díaz2,
  2. Christian Sánchez-Espinel2,
  3. Manuel Arruebo1,*,
  4. José Faro2,3,
  5. Encarnación de Miguel4,
  6. Susana Magadán5,
  7. Clara Yagüe1,
  8. Rodrigo Fernández-Pacheco1,
  9. M. Ricardo Ibarra1,
  10. Jesús Santamaría1,
  11. África González-Fernández2

Article first published online: 15 OCT 2008

DOI: 10.1002/smll.200800199

Issue

Small

Small

Volume 4, Issue 11, pages 2025–2034, November 2008

Additional Information

How to Cite

Díaz, B., Sánchez-Espinel, C., Arruebo, M., Faro, J., de Miguel, E., Magadán, S., Yagüe, C., Fernández-Pacheco, R., Ibarra, M. R., Santamaría, J. and González-Fernández, Á. (2008), Assessing Methods for Blood Cell Cytotoxic Responses to Inorganic Nanoparticles and Nanoparticle Aggregates. Small, 4: 2025–2034. doi: 10.1002/smll.200800199

Author Information

  1. 1

    Aragon Nanoscience Institute (INA) University of Zaragoza Pedro Cerbuna 12, 50009 Zaragoza (Spain)

  2. 2

    Immunology Area, Faculty of Biology University of Vigo Campus As Lagoas–Marcosende 36310 Vigo, Pontevedra (Spain)

  3. 3

    Estudos Avançados de Oeiras Instituto Gulbenkian de Ciência Oeiras (Portugal)

  4. 4

    Cellular Biology Area, Faculty of Biology University of Vigo, Campus As Lagoas–Marcosende 36310 Vigo, Pontevedra (Spain)

  5. 5

    Instituto Superior de Saude do Alto Ave (ISAVE) Quinta de Matos, Geraz do Minho, 4830-31 PVL (Portugal)

*Aragon Nanoscience Institute (INA) University of Zaragoza Pedro Cerbuna 12, 50009 Zaragoza (Spain).

Publication History

  1. Issue published online: 3 NOV 2008
  2. Article first published online: 15 OCT 2008
  3. Manuscript Revised: 20 MAY 2008
  4. Manuscript Received: 4 FEB 2008

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Keywords:

  • cytotoxicity;
  • magnetic nanoparticles;
  • reactive oxygen species;
  • silica

Graphical Abstract

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The cellular uptake of five different nanoparticles (NPs) is investigated in vivo with different types of human peripheral blood cells (lymphocytes, monocytes, granulocytes and erythrocytes), mouse macrophages, and human tumor cell lines (see image). The cytotoxicity, internalization, aggregation in medium, and reactive oxygen species production of the NPs are examined, and differences dependent on both NP and cell type are found.

Abstract

Inorganic nanoparticles (NPs) show great potential for medicinal therapy. However, biocompatibility studies are essential to determine if they are safe. Here, five different NPs are compared for their cytotoxicity, internalization, aggregation in medium, and reactive oxygen species (ROS) production, using tumoral and normal human blood cells. Differences depending on the cell type are analyzed, and no direct correlation between ROS production and cell toxicity is found. Results are discussed with the aim of standardizing the procedures for the evaluation of the toxicity.

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