In vitro Derby Imaging of Cancer Biomarkers Using Quantum Dots

Authors

  • Mee Hyang Ko,

    1. Department of Nuclear Medicine Seoul National University College of Medicine 28 Yongon-dong, Jongno-gu Seoul 110-744 (Republic of Korea)
    Search for more papers by this author
  • Soonhag Kim,

    Corresponding author
    1. Seoul National University College of Medicine 28 Yongon-dong, Jongno-gu Seoul 110-744 (Republic of Korea)
    • Seoul National University College of Medicine 28 Yongon-dong, Jongno-gu Seoul 110-744 (Republic of Korea).
    Search for more papers by this author
  • Won Jun Kang,

    1. Division of Nuclear Medicine, Department of Radiology College of Medicine, Yonsei University Seoul (Republic of Korea)
    Search for more papers by this author
  • Jung Hwan Lee,

    1. Aptamer Unit, Postech Biotech Center Pohang University of Science and Technology Pohang, Kyungbuk (Republic of Korea)
    Search for more papers by this author
  • Hyungu Kang,

    1. Aptamer Unit, Postech Biotech Center Pohang University of Science and Technology Pohang, Kyungbuk (Republic of Korea)
    Search for more papers by this author
  • Sung Hwan Moon,

    1. Aptamer Unit, Postech Biotech Center Pohang University of Science and Technology Pohang, Kyungbuk (Republic of Korea)
    Search for more papers by this author
  • Do Won Hwang,

    1. Department of Nuclear Medicine Seoul National University College of Medicine 28 Yongon-dong, Jongno-gu Seoul 110-744 (Republic of Korea)
    Search for more papers by this author
  • Hae Young Ko,

    1. Department of Nuclear Medicine Seoul National University College of Medicine 28 Yongon-dong, Jongno-gu Seoul 110-744 (Republic of Korea)
    Search for more papers by this author
  • Dong Soo Lee

    Corresponding author
    1. Department of Nuclear Medicine Seoul National University College of Medicine 28 Yongon-dong, Jongno-gu Seoul 110-744 (Republic of Korea)
    • Department of Nuclear Medicine Seoul National University College of Medicine 28 Yongon-dong, Jongno-gu Seoul 110-744 (Republic of Korea).
    Search for more papers by this author

Abstract

Semiconductor quantum dots (QDs), which have broad absorption with narrow emission spectra, are useful for multiplex imaging. Here, fluorescence derby imaging using dual color QDs conjugated by the AS1411 aptamer (targeting nucleolin) and the arginine–glycine–aspartic acid (targeting the integrin αvβ3) in cancer cells is reported. Simultaneous fluorescence imaging of cellular distribution of nucleolin and integrin αvβ3 using QDs enables easy monitoring of separate targets in the cancer cells and the normal healthy cells. These results suggest the feasibility of a concurrent visualization of QD-based multiple cancer biomarkers using small molecules such as aptamer or peptide ligands.

Ancillary