Quantitative CARS Microscopic Detection of Analytes and Their Isotopomers in a Two-Channel Microfluidic Chip


  • Financial support from the German Research Foundation (DFG; SFB 630/TP C1: G.B. and S.S.; Heisenberg Fellowship for S.S.), the Fonds der Chemischen Industrie (B.D.), the Europäischer Fonds für regionale Entwicklung (EFRE), and the Thüringer Kultusministerium (TKM, B578-06001) is acknowledged. CARS=coherent anti-Stokes Raman scattering.


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Fast and quantitative detection of organic analytes is achieved by coherent anti-Stokes Raman scattering (CARS) microscopy using a two-channel microfluidic chip in combination with deuterated isotopomers as a reference (see picture). This experimental design enables the simultaneous measurement of both the chemically relevant Raman-resonant signal and the non-Raman-resonant background.