Intracellular Silicon Chips in Living Cells

Authors

  • Rodrigo Gómez-Martínez,

    Corresponding author
    1. Instituto de Microelectrónica de Barcelona IMB-CNM (CSIC) Campus UAB s/n Cerdanyola, 08193 Barcelona (Spain)
    • Instituto de Microelectrónica de Barcelona IMB-CNM (CSIC) Campus UAB s/n Cerdanyola, 08193 Barcelona (Spain).
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    • R.G.-M. and P.V. contributed equally to this work.

  • Patricia Vázquez,

    Corresponding author
    1. Centro de Investigaciones Biológicas, CIB (CSIC) C/Ramiro de Maeztu 9, 28040 Madrid (Spain)
    • Centro de Investigaciones Biológicas, CIB (CSIC) C/Ramiro de Maeztu 9, 28040 Madrid (Spain).
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    • R.G.-M. and P.V. contributed equally to this work.

  • Marta Duch,

    1. Instituto de Microelectrónica de Barcelona IMB-CNM (CSIC) Campus UAB s/n Cerdanyola, 08193 Barcelona (Spain)
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  • Alejandro Muriano,

    1. Instituto de Investigaciones Químicas y Ambientales de Barcelona IIQAB (CSIC) C/Jorge Girona, 18-26, 08034 Barcelona (Spain)
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  • Daniel Pinacho,

    1. Instituto de Investigaciones Químicas y Ambientales de Barcelona IIQAB (CSIC) C/Jorge Girona, 18-26, 08034 Barcelona (Spain)
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  • Nuria Sanvicens,

    1. Instituto de Investigaciones Químicas y Ambientales de Barcelona IIQAB (CSIC) C/Jorge Girona, 18-26, 08034 Barcelona (Spain)
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  • Francisco Sánchez-Baeza,

    1. Instituto de Investigaciones Químicas y Ambientales de Barcelona IIQAB (CSIC) C/Jorge Girona, 18-26, 08034 Barcelona (Spain)
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  • Patricia Boya,

    1. Centro de Investigaciones Biológicas, CIB (CSIC) C/Ramiro de Maeztu 9, 28040 Madrid (Spain)
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  • Enrique J. de la Rosa,

    1. Centro de Investigaciones Biológicas, CIB (CSIC) C/Ramiro de Maeztu 9, 28040 Madrid (Spain)
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  • Jaume Esteve,

    1. Instituto de Microelectrónica de Barcelona IMB-CNM (CSIC) Campus UAB s/n Cerdanyola, 08193 Barcelona (Spain)
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  • Teresa Suárez,

    Corresponding author
    1. Centro de Investigaciones Biológicas, CIB (CSIC) C/Ramiro de Maeztu 9, 28040 Madrid (Spain)
    • Centro de Investigaciones Biológicas, CIB (CSIC) C/Ramiro de Maeztu 9, 28040 Madrid (Spain).
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    • T.S. and J.A.P. are the project leaders.

  • José A. Plaza

    Corresponding author
    1. Instituto de Microelectrónica de Barcelona IMB-CNM (CSIC) Campus UAB s/n Cerdanyola, 08193 Barcelona (Spain)
    • Instituto de Microelectrónica de Barcelona IMB-CNM (CSIC) Campus UAB s/n Cerdanyola, 08193 Barcelona (Spain).
    Search for more papers by this author
    • T.S. and J.A.P. are the project leaders.


  • This study was financed by the Spanish government through the MINAHE 2 project MEC-TEC2005-07996-CO2-01, MINAHE 3 project MEC-TEC2008-06883-CO3-01, the health research project SAF2007-66175 (to EJdlR), and the INTRACELL project CSIC-200550F0241 8 (to JE, EJdlR & FSB). We acknowledge support (PV) from the CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM). We want to thank Mayte Seisdedos, at the CIB, for her excellent assistance with confocal and time-lapse microscopy. We also thank the MEC-GICSERV program and the IMB-CNM clean-room staff.

Abstract

original image

Silicon-based chips offer high flexibility and versatility as they are based on semiconductor and MEMS technology. They can be top-down fabricated with well-controlled shape and size. Silicon microchips can be internalized into eukaryotic cells (see image) without interfering with cell viability. Engineered intracellular chips with different biomolecules attached to the surface can be used as intracellular sensors.

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