Restriction-Enzyme-Coded Gold-Nanoparticle Probes for Multiplexed DNA Detection

Authors

  • Kyung-Jin Jang,

    1. Interdisciplinary Program in Nano-Science and Technology Seoul National University San 56-1, Sillim-dong, Gwanak-gu Seoul 151-747 (South Korea)
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  • Hyojin Lee,

    1. Department of Chemistry Seoul National University San 56-1, Sillim-dong, Gwanak-gu Seoul 151-747 (South Korea)
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  • Hua-Lan Jin,

    1. Department of Chemistry Seoul National University San 56-1, Sillim-dong, Gwanak-gu Seoul 151-747 (South Korea)
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  • Yeonhu Park,

    1. Department of Chemistry Seoul National University San 56-1, Sillim-dong, Gwanak-gu Seoul 151-747 (South Korea)
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  • Jwa-Min Nam

    Corresponding author
    1. Interdisciplinary Program in Nano-Science and Technology Seoul National University San 56-1, Sillim-dong, Gwanak-gu Seoul 151-747 (South Korea)
    2. Department of Chemistry Seoul National University San 56-1, Sillim-dong, Gwanak-gu Seoul 151-747 (South Korea)
    • Interdisciplinary Program in Nano-Science and Technology Seoul National University San 56-1, Sillim-dong, Gwanak-gu Seoul 151-747 (South Korea).
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  • This work was supported by the Korea Science and Engineering Foundation from the Ministry of Education, Science, and Technology [21C Frontier Functional Proteomics Project (FPR08-A2-150) and Nano R&D program (2008-02890)] and the Seoul R&BD program (11001 and CR070027).

Abstract

original image

A highly sensitive multiplexed DNA detection assay uses restriction-enzyme-encoded, DNA-modified magnetic microparticle and gold nanoparticle probes combined with dark-field nanoparticle imaging analysis (see picture). A sensitivity of 100 fM and high specificity of the detection targets are achieved.

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