This work was supported by the Korea Science and Engineering Foundation from the Ministry of Education, Science, and Technology [21C Frontier Functional Proteomics Project (FPR08-A2-150) and Nano R&D program (2008-02890)] and the Seoul R&BD program (11001 and CR070027).
1613-6829/asset/olbannerleft.gif?v=1&s=abb62bf4c508cccceaedd2e443743ab301acf753)
1613-6829/asset/olbannerright.gif?v=1&s=a0928f6da005e96a3ecfdb9f725fca70c1592474)
Communication
Restriction-Enzyme-Coded Gold-Nanoparticle Probes for Multiplexed DNA Detection†
Article first published online: 31 AUG 2009
DOI: 10.1002/smll.200901105
Copyright © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Jang, K.-J., Lee, H., Jin, H.-L., Park, Y. and Nam, J.-M. (2009), Restriction-Enzyme-Coded Gold-Nanoparticle Probes for Multiplexed DNA Detection. Small, 5: 2665–2668. doi: 10.1002/smll.200901105
- †
Publication History
- Issue published online: 1 DEC 2009
- Article first published online: 31 AUG 2009
- Manuscript Received: 25 JUN 2009
Keywords:
- assays;
- dark-field microscopy;
- DNA;
- nanoparticles;
- restriction enzymes
A highly sensitive multiplexed DNA detection assay uses restriction-enzyme-encoded, DNA-modified magnetic microparticle and gold nanoparticle probes combined with dark-field nanoparticle imaging analysis (see picture). A sensitivity of 100 fM and high specificity of the detection targets are achieved.