This work was supported by the Korea Science and Engineering Foundation from the Ministry of Education, Science, and Technology [21C Frontier Functional Proteomics Project (FPR08-A2-150) and Nano R&D program (2008-02890)] and the Seoul R&BD program (11001 and CR070027).
Restriction-Enzyme-Coded Gold-Nanoparticle Probes for Multiplexed DNA Detection†
Article first published online: 31 AUG 2009
Copyright © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 5, Issue 23, pages 2665–2668, December 4, 2009
How to Cite
Jang, K.-J., Lee, H., Jin, H.-L., Park, Y. and Nam, J.-M. (2009), Restriction-Enzyme-Coded Gold-Nanoparticle Probes for Multiplexed DNA Detection. Small, 5: 2665–2668. doi: 10.1002/smll.200901105
- Issue published online: 1 DEC 2009
- Article first published online: 31 AUG 2009
- Manuscript Received: 25 JUN 2009
- dark-field microscopy;
- restriction enzymes
A highly sensitive multiplexed DNA detection assay uses restriction-enzyme-encoded, DNA-modified magnetic microparticle and gold nanoparticle probes combined with dark-field nanoparticle imaging analysis (see picture). A sensitivity of 100 fM and high specificity of the detection targets are achieved.