Get access

Interface-Directed Self-Assembly of Cell-Laden Microgels

Authors

  • Behnam Zamanian,

    1. Center for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School 65 Landsdowne Street, Cambridge, MA 02139 (USA)
    2. Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology Cambridge, MA 02139 (USA)
    Search for more papers by this author
    • These authors contributed equally to this work.

    • B.Z. and M.M. contributed equally to this work. B.Z., M.M., and A.K. conceived of the project and designed methodology and the experiments. B.Z., M.M., and H.B. performed the experiments and the immunostaining. B.Z., M.M., and M.K. analyzed the data. B.Z., M.M., J.N., and A.K. wrote the manuscript; M.J.H. wrote the theoretical rationale. All authors revised the manuscript and consented to the contents of the final version.

  • Mahdokht Masaeli,

    1. Center for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School 65 Landsdowne Street, Cambridge, MA 02139 (USA)
    2. Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology Cambridge, MA 02139 (USA)
    3. Department of Electrical and Computer Engineering Northeastern University Boston, MA 02115 (USA)
    Search for more papers by this author
    • These authors contributed equally to this work.

    • B.Z. and M.M. contributed equally to this work. B.Z., M.M., and A.K. conceived of the project and designed methodology and the experiments. B.Z., M.M., and H.B. performed the experiments and the immunostaining. B.Z., M.M., and M.K. analyzed the data. B.Z., M.M., J.N., and A.K. wrote the manuscript; M.J.H. wrote the theoretical rationale. All authors revised the manuscript and consented to the contents of the final version.

  • Jason W. Nichol,

    1. Center for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School 65 Landsdowne Street, Cambridge, MA 02139 (USA)
    2. Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology Cambridge, MA 02139 (USA)
    Search for more papers by this author
    • B.Z. and M.M. contributed equally to this work. B.Z., M.M., and A.K. conceived of the project and designed methodology and the experiments. B.Z., M.M., and H.B. performed the experiments and the immunostaining. B.Z., M.M., and M.K. analyzed the data. B.Z., M.M., J.N., and A.K. wrote the manuscript; M.J.H. wrote the theoretical rationale. All authors revised the manuscript and consented to the contents of the final version.

  • Masoud Khabiry,

    1. Center for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School 65 Landsdowne Street, Cambridge, MA 02139 (USA)
    2. Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology Cambridge, MA 02139 (USA)
    Search for more papers by this author
    • B.Z. and M.M. contributed equally to this work. B.Z., M.M., and A.K. conceived of the project and designed methodology and the experiments. B.Z., M.M., and H.B. performed the experiments and the immunostaining. B.Z., M.M., and M.K. analyzed the data. B.Z., M.M., J.N., and A.K. wrote the manuscript; M.J.H. wrote the theoretical rationale. All authors revised the manuscript and consented to the contents of the final version.

  • Matthew J. Hancock,

    1. Center for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School 65 Landsdowne Street, Cambridge, MA 02139 (USA)
    2. Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology Cambridge, MA 02139 (USA)
    Search for more papers by this author
    • B.Z. and M.M. contributed equally to this work. B.Z., M.M., and A.K. conceived of the project and designed methodology and the experiments. B.Z., M.M., and H.B. performed the experiments and the immunostaining. B.Z., M.M., and M.K. analyzed the data. B.Z., M.M., J.N., and A.K. wrote the manuscript; M.J.H. wrote the theoretical rationale. All authors revised the manuscript and consented to the contents of the final version.

  • Hojae Bae,

    1. Center for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School 65 Landsdowne Street, Cambridge, MA 02139 (USA)
    2. Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology Cambridge, MA 02139 (USA)
    Search for more papers by this author
    • B.Z. and M.M. contributed equally to this work. B.Z., M.M., and A.K. conceived of the project and designed methodology and the experiments. B.Z., M.M., and H.B. performed the experiments and the immunostaining. B.Z., M.M., and M.K. analyzed the data. B.Z., M.M., J.N., and A.K. wrote the manuscript; M.J.H. wrote the theoretical rationale. All authors revised the manuscript and consented to the contents of the final version.

  • Ali Khademhosseini

    Corresponding author
    1. Partners Research Building Room 252, 65 Landsdowne Street, Cambridge, MA 02139 (USA)
    2. Center for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School 65 Landsdowne Street, Cambridge, MA 02139 (USA)
    3. Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology Cambridge, MA 02139 (USA)
    • Partners Research Building Room 252, 65 Landsdowne Street, Cambridge, MA 02139 (USA).
    Search for more papers by this author
    • B.Z. and M.M. contributed equally to this work. B.Z., M.M., and A.K. conceived of the project and designed methodology and the experiments. B.Z., M.M., and H.B. performed the experiments and the immunostaining. B.Z., M.M., and M.K. analyzed the data. B.Z., M.M., J.N., and A.K. wrote the manuscript; M.J.H. wrote the theoretical rationale. All authors revised the manuscript and consented to the contents of the final version.


Abstract

Cell-laden hydrogels show great promise for creating engineered tissues. However, a major shortcoming with these systems has been the inability to fabricate structures with controlled micrometer-scale features on a biologically relevant length scale. In this Full Paper, a rapid method is demonstrated for creating centimeter-scale, cell-laden hydrogels through the assembly of shape-controlled microgels or a liquid–air interface. Cell-laden microgels of specific shapes are randomly placed on the surface of a high-density, hydrophobic solution, induced to aggregate and then crosslinked into macroscale tissue-like structures. The resulting assemblies are cell-laden hydrogel sheets consisting of tightly packed, ordered microgel units. In addition, a hierarchical approach creates complex multigel building blocks, which are then assembled into tissues with precise spatial control over the cell distribution. The results demonstrate that forces at an air–liquid interface can be used to self-assemble spatially controllable, cocultured tissue-like structures.

Get access to the full text of this article

Ancillary