Constraint of DNA on Functionalized Graphene Improves its Biostability and Specificity


  • The work performed at Pacific Northwest National Laboratory (PNNL) was supported by DOE's LDRD Program. The work was performed at the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by DOE. PNNL is operated by Battelle for DOE under Contract DE-AC05-76RL01830. I.A.A. acknowledges support from an ARO MURI under Grant No. W911NF-09-1-0476.


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Single-stranded DNA constrained on a graphene surface is effectively protected from enzymatic cleavage by DNase I. Various spectroscopy studies suggest that the single-stranded DNA is promptly adsorbed onto graphene forming strong molecular interactions. Constraint of the DNA probe on the graphene surface improves the specificity of its response to complementary DNA.