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Polymerase-Functionalized Hierarchical Mesoporous Titania Thin Films: Towards a Nanoreactor Platform for DNA Amplification

Authors

  • Martín G. Bellino,

    Corresponding author
    1. Gerencia Química, Comisión Nacional de Energía Atómica Av. Gral. Paz 1499, San Martín, B1650KNA (Argentina)
    • Gerencia Química, Comisión Nacional de Energía Atómica Av. Gral. Paz 1499, San Martín, B1650KNA (Argentina).
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  • Ivanna Tropper,

    1. Departamento de Radiobiología Comisión Nacional de Energía Atómica Av. Gral. Paz 1499, San Martín, B1650KNA (Argentina)
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  • Hebe Duran,

    1. Departamento de Radiobiología Comisión Nacional de Energía Atómica Av. Gral. Paz 1499, San Martín, B1650KNA (Argentina)
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  • Alberto E. Regazzoni,

    1. Gerencia Química, Comisión Nacional de Energía Atómica Av. Gral. Paz 1499, San Martín, B1650KNA (Argentina)
    2. Instituto Jorge A. Sabato Universidad Nacional de San Martín Av. Gral. Paz 1499, San Martín, B1650KNA (Argentina)
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  • Galo J. A. A. Soler-Illia

    Corresponding author
    1. Gerencia Química, Comisión Nacional de Energía Atómica Av. Gral. Paz 1499, San Martín, B1650KNA (Argentina)
    • Gerencia Química, Comisión Nacional de Energía Atómica Av. Gral. Paz 1499, San Martín, B1650KNA (Argentina).
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  • Thanks are due to ANPCyT (PICT-34518, PICT-00335, and PAE-37063/PME-00038) and to LNLS for travel support (projects D10A–XRD2 8260/08 and D11A-SAXS1-8249/08). I.T. acknowledges a fellowship from UNSAM, of which H.D. is a member. A.E.R., H.D., M.G.B., and G.J.A.A.S.-I. are members of CONICET.

Abstract

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Titania thin films with hierarchical nanopore architecture are efficient hosts for Taq polymerase, which is selectively located in large-size mesopores. These biofunctional nanomaterials, highly tuned on the biomolecular length scale, are able to efficiently amplify DNA in a polymerase chain reaction. The engineered pore sizes and interconnectivity at the nanoscale are the key to bio-functionalization and enzyme performance.

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