These authors contributed equally to this work.
Single-Molecule STED Microscopy with Photostable Organic Fluorophores†
Article first published online: 2 JUN 2010
Copyright © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 6, Issue 13, pages 1379–1384, July 5, 2010
How to Cite
Kasper, R., Harke, B., Forthmann, C., Tinnefeld, P., Hell, S. W. and Sauer, M. (2010), Single-Molecule STED Microscopy with Photostable Organic Fluorophores. Small, 6: 1379–1384. doi: 10.1002/smll.201000203
This work was supported by the Biophotonics Program of the BMBF/VDI (grant 13N9234) and the Nanosystems Initiative Munich (NIM). We thank R. Medda for providing labeled cells for bleaching experiments on microtubule networks. STED = stimulated emission depletion.
- Issue published online: 29 JUN 2010
- Article first published online: 2 JUN 2010
- Manuscript Revised: 2 APR 2010
- Manuscript Received: 8 FEB 2010
- Biophotonics Program of the BMBF/VDI. Grant Number: 13N9234
- redox chemistry;
- stimulated emission depletion microscopy
The combination of stimulated emission depletion (STED) microscopy with fluorophore stabilization through a reducing and oxidizing system (ROXS) enables the repetitive measurements necessary for 3D or dynamic STED imaging and resolution enhancement at the single-molecule level. A lateral resolution of <30 nm is obtained for raw image data recorded from single organic fluorophores immobilized in aqueous buffer.