Communication

Single-Molecule STED Microscopy with Photostable Organic Fluorophores†

  1. Robert Kasper4,‡,
  2. Benjamin Harke2,‡,
  3. Carsten Forthmann1,
  4. Philip Tinnefeld1,*,
  5. Stefan W. Hell2,*,
  6. Markus Sauer3,*

Article first published online: 2 JUN 2010

DOI: 10.1002/smll.201000203

Issue

Small

Small

Volume 6, Issue 13, pages 1379–1384, July 5, 2010

Additional Information

How to Cite

Kasper, R., Harke, B., Forthmann, C., Tinnefeld, P., Hell, S. W. and Sauer, M. (2010), Single-Molecule STED Microscopy with Photostable Organic Fluorophores. Small, 6: 1379–1384. doi: 10.1002/smll.201000203

Author Information

  1. 1

    Angewandte Physik–Biophysik and Center for NanoScience Ludwig-Maximilians-University Amalienstr. 54, 80799 Munich (Germany)

  2. 2

    Max Planck Institute for Biophysical Chemistry Am Faßberg 11, 37077 Göttingen (Germany)

  3. 3

    Biotechnology and Biophysics Julius-Maximilians-University Wuerzburg Am Hubland, 97074 Wuerzburg (Germany)

  4. 4

    Applied Laser Physics and Laser Spectroscopy Bielefeld University Universitätsstr. 25, 33615 Bielefeld (Germany)

  1. ‡

    These authors contributed equally to this work.

*Angewandte Physik–Biophysik and Center for NanoScience Ludwig-Maximilians-University Amalienstr. 54, 80799 Munich (Germany)

  1. †

    This work was supported by the Biophotonics Program of the BMBF/VDI (grant 13N9234) and the Nanosystems Initiative Munich (NIM). We thank R. Medda for providing labeled cells for bleaching experiments on microtubule networks. STED = stimulated emission depletion.

  2. ‡

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 29 JUN 2010
  2. Article first published online: 2 JUN 2010
  3. Manuscript Revised: 2 APR 2010
  4. Manuscript Received: 8 FEB 2010

Funded by

  • Biophotonics Program of the BMBF/VDI. Grant Number: 13N9234

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Keywords:

  • fluorescence;
  • imaging;
  • photostability;
  • redox chemistry;
  • stimulated emission depletion microscopy
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The combination of stimulated emission depletion (STED) microscopy with fluorophore stabilization through a reducing and oxidizing system (ROXS) enables the repetitive measurements necessary for 3D or dynamic STED imaging and resolution enhancement at the single-molecule level. A lateral resolution of <30 nm is obtained for raw image data recorded from single organic fluorophores immobilized in aqueous buffer.

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