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Keywords:

  • fluorescence;
  • imaging;
  • photostability;
  • redox chemistry;
  • stimulated emission depletion microscopy
Thumbnail image of graphical abstract

The combination of stimulated emission depletion (STED) microscopy with fluorophore stabilization through a reducing and oxidizing system (ROXS) enables the repetitive measurements necessary for 3D or dynamic STED imaging and resolution enhancement at the single-molecule level. A lateral resolution of <30 nm is obtained for raw image data recorded from single organic fluorophores immobilized in aqueous buffer.