Intracellular Imaging with a Graphene-Based Fluorescent Probe

Authors

  • Cheng Peng,

    1. Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (P. R. China)
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  • Wenbing Hu,

    1. Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (P. R. China)
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  • Yuntao Zhou,

    1. Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (P. R. China)
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  • Chunhai Fan,

    Corresponding author
    1. Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (P. R. China)
    • Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (P. R. China).
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  • Qing Huang

    Corresponding author
    1. Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (P. R. China)
    • Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (P. R. China).
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Abstract

Graphene is an increasingly important nanomaterial that has shown great promise in the area of nanotechnology. In this study, fluorescein-functionalized graphene oxide (GO) is synthesized via a polyethylene glycol (PEG) bridge and its application in intracellular imaging is explored. GO is an oxide form of graphene that provides an ideal platform to prepare graphene-based functional nanomaterials via chemical modification. The PEG bridge was introduced to prevent GO-induced quenching of conjugated fluorescein. The fluorescein–PEG–GO conjugate thus prepared exhibits excellent pH-tunable fluorescent properties and, more significantly, can be efficiently taken up by cells and serve as a fluorescent nanoprobe for intracellular imaging.

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