Fabrication of Polymersomes using Double-Emulsion Templates in Glass-Coated Stamped Microfluidic Devices

Authors

  • Julian Thiele,

    1. Department of Physics and School of Engineering and Applied Sciences Harvard University Cambridge, MA 02138 (USA)
    2. Institute of Physical Chemistry University of Hamburg Hamburg 20146 (Germany)
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  • Adam R. Abate,

    1. Department of Physics and School of Engineering and Applied Sciences Harvard University Cambridge, MA 02138 (USA)
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  • Ho Cheung Shum,

    1. Department of Physics and School of Engineering and Applied Sciences Harvard University Cambridge, MA 02138 (USA)
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  • Simone Bachtler,

    1. Department of Physics and School of Engineering and Applied Sciences Harvard University Cambridge, MA 02138 (USA)
    2. Hochschule Mannheim University of Applied Sciences Mannheim 68163 (Germany)
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  • Stephan Förster,

    1. Institute of Physical Chemistry University of Hamburg Hamburg 20146 (Germany)
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  • David A. Weitz

    Corresponding author
    1. Department of Physics and School of Engineering and Applied Sciences Harvard University Cambridge, MA 02138 (USA)
    • Department of Physics and School of Engineering and Applied Science, Harvard University, Cambridge, MA 02138 (USA).
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  • This work was supported by the NSF (DMR-0602684) and the Harvard MRSEC (DMR-0820484). We thank Sebastian Seiffert for assistance with confocal microscopy and helpful discussions, and Kisun Yoon for assistance with SEM. This work was performed in part at the Center for Nanoscale Systems (CNS), which is supported by the NSF (ECS-0335765). JT received funding from the German Academic Exchange Service (D/08/44750) and the Fund of the Chemical Industry (Germany), which is gratefully acknowledged.

Abstract

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The formation of polymersomes from copolymer-stabilized double emulsions in glass-coated, PDMS microfluidic devices is described. The device geometry enables separate injection of two organic solvents to form the shell phase of the double emulsion. This allows proper combination of the two solvents, which is essential for forming polymersomes.

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