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Rapid Prototyping of Nanofluidic Systems Using Size-Reduced Electrospun Nanofibers for Biomolecular Analysis

Authors

  • Seung-min Park,

    1. School of Applied and Engineering Physics, Cornell University, 205 Clark Hall, Ithaca, NY 14853, USA
    Current affiliation:
    1. Current Address: Biomolecular Nanotechnology Center, Berkeley Sensor and Actuator Center, Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA
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  • Yun Suk Huh,

    1. Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14853, USA
    Current affiliation:
    1. Current Address: Division of Materials Science, Korea Basic Science Institute, Daejeon, 305-333, South Korea
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  • Kylan Szeto,

    1. School of Applied and Engineering Physics, Cornell University, 205 Clark Hall, Ithaca, NY 14853, USA
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  • Daniel J. Joe,

    1. School of Electrical and Computer Engineering, Cornell University, Ithaca, NY 14853, USA
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  • Jun Kameoka,

    1. Department of Electrical and Computer Engineering, Texas A&M University, USA
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  • Geoffrey W. Coates,

    1. Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
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  • Joshua B. Edel,

    1. Department of Chemistry and Institute of Biomedical Engineering, Imperial College London, SW7 2AZ, London, UK
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  • David Erickson,

    1. Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14853, USA
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  • Harold G. Craighead

    Corresponding author
    1. School of Applied and Engineering Physics, Cornell University, 205 Clark Hall, Ithaca, NY 14853, USA
    • School of Applied and Engineering Physics, Cornell University, 205 Clark Hall, Ithaca, NY 14853, USA.
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Abstract

Biomolecular transport in nanofluidic confinement offers various means to investigate the behavior of biomolecules in their native aqueous environments, and to develop tools for diverse single-molecule manipulations. Recently, a number of simple nanofluidic fabrication techniques has been demonstrated that utilize electrospun nanofibers as a backbone structure. These techniques are limited by the arbitrary dimension of the resulting nanochannels due to the random nature of electrospinning. Here, a new method for fabricating nanofluidic systems from size-reduced electrospun nanofibers is reported and demonstrated. As it is demonstrated, this method uses the scanned electrospinning technique for generation of oriented sacrificial nanofibers and exposes these nanofibers to harsh, but isotropic etching/heating environments to reduce their cross-sectional dimension. The creation of various nanofluidic systems as small as 20 nm is demonstrated, and practical examples of single biomolecular handling, such as DNA elongation in nanochannels and fluorescence correlation spectroscopic analysis of biomolecules passing through nanochannels, are provided.

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