Dynamic Visualization of RGD-Quantum Dot Binding to Tumor Neovasculature and Extravasation in Multiple Living Mouse Models Using Intravital Microscopy

Authors

  • Bryan Ronain Smith,

    1. The Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Bio-X Program, and Department of Bioengineering, Stanford University School of Medicine, The James H. Clark Center, 318 Campus Drive, E-150, East Wing, 1st Floor, Stanford, CA 94305–5427, USA
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  • Zhen Cheng,

    1. The Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Bio-X Program, and Department of Bioengineering, Stanford University School of Medicine, The James H. Clark Center, 318 Campus Drive, E-150, East Wing, 1st Floor, Stanford, CA 94305–5427, USA
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  • Abhijit De,

    1. The Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Bio-X Program, and Department of Bioengineering, Stanford University School of Medicine, The James H. Clark Center, 318 Campus Drive, E-150, East Wing, 1st Floor, Stanford, CA 94305–5427, USA
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  • Jarrett Rosenberg,

    1. The Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Bio-X Program, and Department of Bioengineering, Stanford University School of Medicine, The James H. Clark Center, 318 Campus Drive, E-150, East Wing, 1st Floor, Stanford, CA 94305–5427, USA
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  • Sanjiv Sam Gambhir

    Corresponding author
    1. The Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Bio-X Program, and Department of Bioengineering, Stanford University School of Medicine, The James H. Clark Center, 318 Campus Drive, E-150, East Wing, 1st Floor, Stanford, CA 94305–5427, USA
    • The Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Bio-X Program, and Department of Bioengineering, Stanford University School of Medicine, The James H. Clark Center, 318 Campus Drive, E-150, East Wing, 1st Floor, Stanford, CA 94305–5427, USA.
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Abstract

original image

The behavior of targeted quantum dots (Qdots) across three different tumor models using intravital microscopy with submicrometer resolution is described. As in the figure, the differences in extravasation between tumor types are shown and the kinetics are quatnified. Further, by demonstrating similarity in Qdot binding to tumor blood vessels across different tumor types, this work suggests several advantages implicit in vascular targeting compared with tumor cell targeting.

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