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Viral Coat Proteins as Flexible Nano-Building-Blocks for Nanoparticle Encapsulation

Authors

  • Feng Li,

    1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No.44, Xiaohongshan, Wuhan 430071, P. R. China
    2. Graduate School, Chinese Academy of Sciences, No.19, Yuquan Road, Shijingshan, Beijing 100049, P. R. China
    Current affiliation:
    1. Current address: Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou 215125, P. R. China
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  • Ke Li,

    1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No.44, Xiaohongshan, Wuhan 430071, P. R. China
    2. Graduate School, Chinese Academy of Sciences, No.19, Yuquan Road, Shijingshan, Beijing 100049, P. R. China
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  • Zong-Qiang Cui,

    1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No.44, Xiaohongshan, Wuhan 430071, P. R. China
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  • Zhi-Ping Zhang,

    1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No.44, Xiaohongshan, Wuhan 430071, P. R. China
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  • Hong-Ping Wei,

    1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No.44, Xiaohongshan, Wuhan 430071, P. R. China
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  • Ding Gao,

    1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No.44, Xiaohongshan, Wuhan 430071, P. R. China
    2. Graduate School, Chinese Academy of Sciences, No.19, Yuquan Road, Shijingshan, Beijing 100049, P. R. China
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  • Jiao-Yu Deng,

    1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No.44, Xiaohongshan, Wuhan 430071, P. R. China
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  • Xian-En Zhang

    Corresponding author
    1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No.44, Xiaohongshan, Wuhan 430071, P. R. China
    • State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No.44, Xiaohongshan, Wuhan 430071, P. R. China.
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Abstract

Viral capsid–nanoparticle hybrid structures offer new opportunities for nanobiotechnology. We previously generated virus-based nanoparticles (VNPs) of simian virus 40 (SV40) containing quantum dots (QDs) for cellular imaging. However, as an interesting issue of nano-bio interfaces, the mechanism of nanoparticle (NP) encapsulation by viral coat proteins remains unclear. Here, four kinds of QDs with the same core/shell but different surface coatings are tested for encapsulation. All the QDs can be encapsulated efficiently and there is no correlation between the encapsulation efficiency and the surface charge of the QDs. All the SV40 VNPs encapsulating differently modified QDs show similar structures, fluorescence properties, and activity in entering living cells. These results demonstrate the flexibility of SV40 major capsid protein VP1 in NP encapsulation and provide new clues to the mechanism of NP packaging by viral shells.

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