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Thin Multilayer Films and Microcapsules Containing DNA Quadruplex Motifs

  1. Francesca Cavalieri2,
  2. Sher Leen Ng1,
  3. Claudia Mazzuca2,
  4. Zhongfan Jia3,
  5. Volga Bulmus3,
  6. Thomas P. Davis3,
  7. Frank Caruso1,*

Article first published online: 23 NOV 2010

DOI: 10.1002/smll.201001246

Issue

Small

Small

Volume 7, Issue 1, pages 101–111, January 3, 2011

Additional Information

How to Cite

Cavalieri, F., Ng, S. L., Mazzuca, C., Jia, Z., Bulmus, V., Davis, T. P. and Caruso, F. (2011), Thin Multilayer Films and Microcapsules Containing DNA Quadruplex Motifs. Small, 7: 101–111. doi: 10.1002/smll.201001246

Author Information

  1. 1

    Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia

  2. 2

    Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, 00173 Rome, Italy

  3. 3

    Centre for Advanced Macromolecular Design, School of Chemical Engineering, The University of New South Wales, Sydney, NSW 2052, Australia

*Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.

Publication History

  1. Issue published online: 27 DEC 2010
  2. Article first published online: 23 NOV 2010
  3. Manuscript Revised: 20 OCT 2010
  4. Manuscript Received: 23 JUL 2010

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Keywords:

  • DNA;
  • self-assembly;
  • layer-by-layer;
  • microcapsules;
  • polymers

Abstract

The assembly of multifunctional nanostructures bearing G-quadruplex motifs broadens the prospects of using G-quadruplexes as therapeutic carriers. Herein, we report the synthesis and characterization of an oligodeoxyguanosine, G15-mer polymer conjugate. We demonstrate that G15-mer oligonucleotides grafted to a polymer chain preserve the ability to self-assemble into ordered structures. The G-quadruplex-polymer conjugates were assembled onto a surface via hybridization with 30-mer cytosine strands, C30-mer, using a layer-by-layer approach to form microcapsules. A mechanism for the sequential assembly of the multilayer films and microcapsules is presented. We further investigate the photophysical behavior of porphyrin TMPyP4 bound to multilayer-coated particles. This study shows that the multilayer films bear residual and functional quadruplex moieties that can be used to effectively bind therapeutic agents.

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