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Intact Mammalian Cell Function on Semiconductor Nanowire Arrays: New Perspectives for Cell-Based Biosensing

Authors

  • Trine Berthing,

    1. Bionanotechnology and Nanomedicine Laboratory, Department of Neuroscience and Pharmacology & Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, Denmark
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  • Sara Bonde,

    1. Bionanotechnology and Nanomedicine Laboratory, Department of Neuroscience and Pharmacology & Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, Denmark
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  • Claus B. Sørensen,

    1. Niels Bohr Institute & Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, Denmark
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  • Pawel Utko,

    1. Niels Bohr Institute & Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, Denmark
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  • Jesper Nygård,

    1. Niels Bohr Institute & Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, Denmark
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  • Karen L. Martinez

    Corresponding author
    1. Bionanotechnology and Nanomedicine Laboratory, Department of Neuroscience and Pharmacology & Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, Denmark
    • Bionanotechnology and Nanomedicine Laboratory, Department of Neuroscience and Pharmacology & Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, Denmark.
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Abstract

Nanowires (NWs) are attracting more and more interest due to their potential cellular applications, such as delivery of compounds or sensing platforms. Arrays of vertical indium-arsenide (InAs) NWs are interfaced with human embryonic kidney cells and rat embryonic dorsal root ganglion neurons. A selection of critical cell functions and pathways are shown not to be impaired, including cell adhesion, membrane integrity, intracellular enzyme activity, DNA uptake, cytosolic and membrane protein expression, and the neuronal maturation pathway. The results demonstrate the low invasiveness of InAs NW arrays, which, combined with the unique physical properties of InAs, open up their potential for cellular investigations.

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