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Visualizing Low-Level Point Mutations: Enzyme-like Selectivity Offered by Nanoparticle Probes

Authors

  • Yanbing Zu,

    Corresponding author
    1. Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore 138669
    • Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore 138669.
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  • Aik Leong Ting,

    1. Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore 138669
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  • Zhiqiang Gao

    Corresponding author
    1. Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore 138669
    2. Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543
    • Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore 138669.
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Abstract

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Highly selective DNA probes prepared by functionalizing gold nanoparticles with nonionic morpholino oligos are described. The nanoparticle probes offer enzyme-like selectivity based simply on hybridization reactions, allowing visual detection of a perfectly matched target within a large excess (>1000-fold) of single-base-mismatched DNA.

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