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Keywords:

  • aptamers;
  • polyelectrolytes;
  • microcapsules;
  • layer-by-layer;
  • controlled release

Abstract

Aptamer–polyelectrolyte microcapsules are prepared for potential use as triggered delivery vehicles and microreactors. The hollow microcapsules are prepared from the sulforhodamine B aptamer and the polyelectrolytes poly(allylamine hydrochloride) and poly(sodium 4-styrene-sulfonate), using layer-by-layer (LbL) film deposition templated on a sacrificial CaCO3 spherical core. Scanning electron microscopy and confocal microscopy confirm the formation of spherical CaCO3 cores and LbL-aptamer microcapsules. Colocalization studies with fluorescently-tagged aptamer and sulforhodamine B verify the ability of the aptamer to recognize its cognate target in the presence of the K+ ions that are required for its characteristic G-quadruplex formation. Fluorescence recovery after photobleaching studies confirms a significant difference in the permeability of the aptamer–polyelectrolyte microcapsules for the sulforhodamine B dye target compared to control microcapsules prepared with a random oligonucleotide. These results suggest that aptamer-based ‘smart’ responsive films and microcapsules could be applied to problems of catalysis and controlled release.