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Label-Free Indirect Immunoassay Using an Avidin-Induced Surface-Enhanced Raman Scattering Substrate

Authors

  • Xiao Xia Han,

    1. Department of Chemistry and Research Center for Single Molecule, Vibrational Spectroscopy, School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo 669–1337, Japan
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  • Lei Chen,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, PR China
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  • Wei Ji,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, PR China
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  • Yunfei Xie,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, PR China
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  • Bing Zhao,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, PR China
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  • Yukihiro Ozaki

    Corresponding author
    1. Department of Chemistry and Research Center for Single Molecule, Vibrational Spectroscopy, School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo 669–1337, Japan
    • Department of Chemistry and Research Center for Single Molecule, Vibrational Spectroscopy, School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo 669–1337, Japan.
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Abstract

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A label-free immunoassay based on a simple, surface-enhanced Raman scattering (SERS) approach is reported. Biotin-conjugated antibodies are at­­tached to avidin-aggregated Ag nanoparticles via biotin–avidin biorecognition. More highly sensitive protein detection and more biocompatible protein adsorption are achieved compared to previous relevant methods. Importantly, a SERS intensity ratio of two bands is found to be sensitive to the concentration of corresponding antigens.

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