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Bio-inspired Micropatterned Platform to Steer Stem Cell Differentiation

Authors

  • Chor Yong Tay,

    1. School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798
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  • Mintu Pal,

    1. School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798
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  • Haiyang Yu,

    1. School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798
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  • Wen Shing Leong,

    1. School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798
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  • Nguan Soon Tan,

    1. School of Biological Sciences Nanyang Technological University 60 Nanyang Drive, Singapore 637551, Singapore
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  • Kee Woei Ng,

    1. School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798
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  • Subbu Venkatraman,

    1. School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798
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  • Freddy Boey,

    1. School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798
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  • David Tai Leong,

    Corresponding author
    1. Department of Chemical and Biomolecular Engineering National University of Singapore 4 Engineering Drive 4 Singapore 117576, Singapore
    2. Cancer Science Institute of Singapore National University of Singapore Centre for Life Science 28 Medical Drive, Singapore 117456, Singapore
    • Department of Chemical and Biomolecular Engineering National University of Singapore 4 Engineering Drive 4 Singapore 117576, Singapore
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  • Lay Poh Tan

    Corresponding author
    1. School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798
    • School of Materials Science and Engineering Nanyang Technological University 50 Nanyang Avenue, Singapore 639798.
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Abstract

original image

Controlled stem cell differentiation on micropatterned biodegradable substrates involves modulation of the canonical FAK–ERK (focal adhesion kinase–extracellular-signal regulated kinase) signaling pathway in the absence of exogenous soluble factors. The use of a micropatterned platform to coerce human mesenchymal stem cells to adopt an elongated morphology bypasses the need to use pharmacological factors to repress FAK–ERK signaling activity to bring about substantial myogenesis.

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