Energy transfer: Visualizing Resonance Energy Transfer in Supramolecular Surface Patterns of β-CD-Functionalized Quantum Dot Hosts and Organic Dye Guests by Fluorescence Lifetime Imaging (Small 24/2010)

Authors

  • Denis Dorokhin,

    1. Materials Science and Technology of Polymers, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands, Fax: +31 53 4893823
    2. Supramolecular Chemistry and Technology, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands, Fax: +31 53 4894645
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  • Shu-Han Hsu,

    1. Supramolecular Chemistry and Technology, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands, Fax: +31 53 4894645
    2. Molecular NanoFabrication, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands
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  • Nikodem Tomczak,

    1. Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602
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  • Christian Blum,

    1. Nanobiophysics, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands
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  • Vinod Subramaniam,

    1. Nanobiophysics, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands
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  • Jurriaan Huskens,

    1. Molecular NanoFabrication, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands
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  • David N. Reinhoudt,

    1. Supramolecular Chemistry and Technology, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands, Fax: +31 53 4894645
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  • Aldrik H. Velders,

    Corresponding author
    1. Supramolecular Chemistry and Technology, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands, Fax: +31 53 4894645
    • Supramolecular Chemistry and Technology, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands, Fax: +31 53 4894645.
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  • G. Julius Vancso

    Corresponding author
    1. Materials Science and Technology of Polymers, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands, Fax: +31 53 4893823
    • Materials Science and Technology of Polymers, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands, Fax: +31 53 4893823
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Abstract

Detection of an analyte via supramolecular host–guest binding and quantum dot (QD)-based fluorescence resonance energy transfer (FRET) signal transduction mechanism is demonstrated. Surface patterns consisting of CdSe/ZnS QDs functionalized at their periphery with β-cyclodextrin (β-CD) were obtained by immobilization of the QDs from solution onto glass substrates patterned with adamantyl-terminated poly(propylene imine) dendrimeric “glue.” Subsequent formation of host–guest complexes between vacant β-CD on the QD surface and an adamantyl-functionalized lissamine rhodamine resulting in FRET was confirmed by fluorescence microscopy, spectroscopy, and fluorescence lifetime imaging microscopy (FLIM).

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