Nano-C60: A Novel, Effective, Fluorescent Sensing Platform for Biomolecular Detection

Authors

  • Hailong Li,

    1. State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun 130022, Jilin, P.R. China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, P.R. China
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  • Yingwei Zhang,

    1. State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun 130022, Jilin, P.R. China
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  • Yonglan Luo,

    1. State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun 130022, Jilin, P.R. China
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  • Xuping Sun

    Corresponding author
    1. State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun 130022, Jilin, P.R. China
    • State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun 130022, Jilin, P.R. China.
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Abstract

Water-soluble nano-C60 can serve as a novel, effective, fluorescent sensing platform for biomolecular detection with high sensitivity and selectivity. In this paper, fluorescent detection of DNA and thrombin via nano-C60 is demonstrated for the first time. The principle of the assay lies in the fact that the adsorption of the fluorescently labeled single-stranded DNA (ssDNA) probe by nano-C60 leads to substantial fluorescence quenching. In the presence of a target, the biomolecular mutual interaction suppresses this quenching, signaling the existence of the target. This sensing system rivals graphene oxide but is superior to other carbon-structure-based systems. The present method can also achieve multiplex DNA detection and withstand the interference from human blood serum.

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