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Site-Specific Attachment of Proteins onto a 3D DNA Tetrahedron through Backbone-Modified Phosphorothioate DNA

Authors

  • Ngo Yin Wong,

    1. Material Science and Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
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  • Chuan Zhang,

    1. Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA
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  • Li Huey Tan,

    1. Department of Chemistry, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
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  • Yi Lu

    Corresponding author
    1. Material Science and Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
    2. Department of Chemistry, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
    • Material Science and Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
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Abstract

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Phosphorothioate-modified DNA and short bifunctional linkers are used to attach proteins to a DNA tetrahedron. Precise control of the position of and distance between protein modifications is achieved. This technique is simple, can be adapted to other DNA nanostructures, exhibits high yields, and the modification can be programmably placed within the structure.

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