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Wet Nanoscale Imaging and Testing of Polymersomes

Authors

  • Giuseppe Battaglia,

    Corresponding author
    1. The Krebs Institute, Department of Biomedical Science, The University of Sheffield, Sheffield, S10 2TN, UK
    • The Krebs Institute, Department of Biomedical Science, The University of Sheffield, Sheffield, S10 2TN, UK.
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  • Caterina LoPresti,

    1. The Krebs Institute, Department of Biomedical Science, The University of Sheffield, Sheffield, S10 2TN, UK
    2. The Kroto Research Institute, Department of Materials Science and Engineering, The University of Sheffield, Sheffield, S3 7HF, UK
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  • Marzia Massignani,

    1. The Krebs Institute, Department of Biomedical Science, The University of Sheffield, Sheffield, S10 2TN, UK
    2. The Kroto Research Institute, Department of Materials Science and Engineering, The University of Sheffield, Sheffield, S3 7HF, UK
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  • Nicholas J. Warren,

    1. The Krebs Institute, Department of Biomedical Science, The University of Sheffield, Sheffield, S10 2TN, UK
    2. Department of Chemistry, The University of Sheffield, Sheffield, S3 7HF, UK
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  • Jeppe Madsen,

    1. The Krebs Institute, Department of Biomedical Science, The University of Sheffield, Sheffield, S10 2TN, UK
    2. Department of Chemistry, The University of Sheffield, Sheffield, S3 7HF, UK
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  • Simon Forster,

    1. The Kroto Research Institute, Department of Materials Science and Engineering, The University of Sheffield, Sheffield, S3 7HF, UK
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  • Cvetilin Vasilev,

    1. The Krebs Institute, Department of Physics and Astronomy, The University of Sheffield, Sheffield, S10 2TN, UK
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  • Jamie K. Hobbs,

    1. The Krebs Institute, Department of Physics and Astronomy, The University of Sheffield, Sheffield, S10 2TN, UK
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  • Steven P. Armes,

    1. Department of Chemistry, The University of Sheffield, Sheffield, S3 7HF, UK
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  • Somoyot Chirasatitsin,

    1. Department of Bioengineering, University of California San Diego, La Jolla, USA
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  • Adam J. Engler

    1. Department of Bioengineering, University of California San Diego, La Jolla, USA
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Abstract

original image

A facile methodology for the immobilization of polymersomes is demo­nstrated using ad-hoc synthesized, biotinylated copolymers or commercially available biotinylated phospholipids on streptavidin-decorated substrates. This allows the imaging of polymersomes using high-resolution techniques such as stimulated emission depletion microscopy and atomic force microscopy. This latter allows the gathering of other important functional parameters such as mechanical properties and surface topology, enabling characterization of these water-borne nanoparticles in wet conditions and hence in their natural environment.

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