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Selective Nanopatterning of Protein via Ion-Induced Focusing and its Application to Metal-Enhanced Fluorescence

Authors

  • Chang Gyu Woo,

    1. National CRI Center for Nano Particle Control, Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151–742, Korea, Fax: (+82) 2 878 2465
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  • Hyuck Shin,

    1. National CRI Center for Nano Particle Control, Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151–742, Korea, Fax: (+82) 2 878 2465
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  • Changui Jeong,

    1. National CRI Center for Nano Particle Control, Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151–742, Korea, Fax: (+82) 2 878 2465
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  • Kimin Jun,

    1. National CRI Center for Nano Particle Control, Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151–742, Korea, Fax: (+82) 2 878 2465
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  • Jungpyo Lee,

    1. National CRI Center for Nano Particle Control, Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151–742, Korea, Fax: (+82) 2 878 2465
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  • Jung-Rok Lee,

    1. National CRI Center for Nano Particle Control, Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151–742, Korea, Fax: (+82) 2 878 2465
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  • Heechul Lee,

    1. National CRI Center for Nano Particle Control, Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151–742, Korea, Fax: (+82) 2 878 2465
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  • Sukbeom You,

    1. National CRI Center for Nano Particle Control, Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151–742, Korea, Fax: (+82) 2 878 2465
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  • Youngsook Son,

    1. Department of Genetic Engineering, Kyung Hee University, Yong In, 446–701, Korea
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  • Mansoo Choi

    Corresponding author
    1. National CRI Center for Nano Particle Control, Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151–742, Korea, Fax: (+82) 2 878 2465
    • National CRI Center for Nano Particle Control, Division of WCU Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, 151–742, Korea, Fax: (+82) 2 878 2465.
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Abstract

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Protein micro/nanoarrays with selectivity can be fabricated in a parallel way through the ion-induced focusing of a charged aerosol form of the proteins. This method can also position protein nanoparticles onto the deep bottom surface within microchannels of a high aspect ratio. Particle trajectory calculations validate the experimental results. A new design of metal-enhanced fluorescence substrate is successfully demonstrated.

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