Hybridization-Induced “Off-On” 19F-NMR Signal Probe Release from DNA-Functionalized Gold Nanoparticles

Authors

  • Alexander Kieger,

    1. Northwestern University, Feinberg School of Medicine, 303 East Chicago Avenue, Chicago, IL 60611-3008, USA
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  • Michael J. Wiester,

    1. Department of Chemistry and International, Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA
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  • Daniel Procissi,

    1. Department of Radiology, Northwestern University, Feinberg School of Medicine, 737 North Michigan Ave, Chicago, IL 60611-3008, USA
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  • Todd B. Parrish,

    1. Department of Radiology, Northwestern University, Feinberg School of Medicine, 737 North Michigan Ave, Chicago, IL 60611-3008, USA
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  • Chad A. Mirkin,

    Corresponding author
    1. Department of Chemistry and International, Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA
    • Department of Chemistry and International, Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA
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  • C. Shad Thaxton

    Corresponding author
    1. Department of Urology, International Institute for Nanotechnology and, Institute for BioNanotechnology and Medicine, Northwestern University, Feinberg School of Medicine, 303 East Chicago Avenue, Chicago, IL 60611-3008, USA
    • Department of Urology, International Institute for Nanotechnology and, Institute for BioNanotechnology and Medicine, Northwestern University, Feinberg School of Medicine, 303 East Chicago Avenue, Chicago, IL 60611-3008, USA.
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Abstract

original image

Fluorine-19-DNA-functionalized gold nanoparticles (19F-DNA AuNPs) are demonstrated to provide a 19F-NMR off–on switch in response to target-specific DNA hybridization events. AuNP-bound 19F-DNA produces a low intensity signal that is undetectable above background. Upon complementary DNA hybridization and subsequent19F-DNA release, the 19F-NMR signal becomes detectable. This method has the potential to be used both in vitro and in vivo to non-invasively detect and image specific nucleic acid binding events of interest.

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