Nanodiamond as a Vector for siRNA Delivery to Ewing Sarcoma Cells

Authors

  • Anna Alhaddad,

    1. Université Paris-Sud, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, F-91405 Orsay, France
    2. CNRS, Orsay, Laboratoire de Vectorologie et, Thérapeutiques Anticancéreuses, UMR 8203, F-91405, France
    3. Institut Gustave Roussy, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, F-94805 Villejuif, France
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  • Marie-Pierre Adam,

    1. Laboratoire de Photonique Quantique et Moléculaire, CNRS & Ecole Normale Supérieure de Cachan - UMR 8537, F-94235 Cachan cedex, France
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  • Jacques Botsoa,

    1. Laboratoire de Photonique Quantique et Moléculaire, CNRS & Ecole Normale Supérieure de Cachan - UMR 8537, F-94235 Cachan cedex, France
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  • Géraldine Dantelle,

    1. Laboratoire de Physique de la Matière Condensée, CNRS & Ecole Polytechnique - UMR 7643, F-91120 Palaiseau, France
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  • Sandrine Perruchas,

    1. Laboratoire de Physique de la Matière Condensée, CNRS & Ecole Polytechnique - UMR 7643, F-91120 Palaiseau, France
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  • Thierry Gacoin,

    1. Laboratoire de Physique de la Matière Condensée, CNRS & Ecole Polytechnique - UMR 7643, F-91120 Palaiseau, France
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  • Christelle Mansuy,

    1. UPMC Paris 6, Laboratoire des BioMolécules, UMR 7203, Université, Pierre et Marie Curie, F-75005 Paris, France
    2. ENS, Département de Chimie, Laboratoire des BioMolécules, UMR 7203, Ecole Normale Supérieure, F-75231 Paris cedex, France
    3. CNRS, Laboratoire des BioMolécules, UMR 7203, F-75231 Paris cedex, France
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  • Solange Lavielle,

    1. UPMC Paris 6, Laboratoire des BioMolécules, UMR 7203, Université, Pierre et Marie Curie, F-75005 Paris, France
    2. ENS, Département de Chimie, Laboratoire des BioMolécules, UMR 7203, Ecole Normale Supérieure, F-75231 Paris cedex, France
    3. CNRS, Laboratoire des BioMolécules, UMR 7203, F-75231 Paris cedex, France
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  • Claude Malvy,

    1. Université Paris-Sud, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, F-91405 Orsay, France
    2. CNRS, Orsay, Laboratoire de Vectorologie et, Thérapeutiques Anticancéreuses, UMR 8203, F-91405, France
    3. Institut Gustave Roussy, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, F-94805 Villejuif, France
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  • François Treussart,

    Corresponding author
    1. Laboratoire de Photonique Quantique et Moléculaire, CNRS & Ecole Normale Supérieure de Cachan - UMR 8537, F-94235 Cachan cedex, France
    • Laboratoire de Photonique Quantique et Moléculaire, CNRS & Ecole Normale Supérieure de Cachan - UMR 8537, F-94235 Cachan cedex, France.
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  • Jean-Rémi Bertrand

    Corresponding author
    1. Université Paris-Sud, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, F-91405 Orsay, France
    2. CNRS, Orsay, Laboratoire de Vectorologie et, Thérapeutiques Anticancéreuses, UMR 8203, F-91405, France
    3. Institut Gustave Roussy, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, F-94805 Villejuif, France
    • Institut Gustave Roussy, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, F-94805 Villejuif, France
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Errata

This article is corrected by:

  1. Errata: Corrigendum: Nanodiamond as a Vector for siRNA Delivery to Ewing Sarcoma Cells Volume 8, Issue 23, 3549, Article first published online: 3 December 2012

Abstract

The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in-vivo anticancer nucleic-acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs’ intrinsic fluorescence from embedded color-center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli-1 gene expression is shown at the mRNA and protein level by the ND-vectorized siRNA in a serum-containing medium.

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