Targeted Delivery of Polyoxometalate Nanocomposites

Authors

  • Georg Geisberger,

    1. Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland, Fax: (+) 41 44 635 6802
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  • Susann Paulus,

    1. Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland, Fax: (+) 41 44 635 6802
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  • Emina Besic Gyenge,

    1. Institute of Anatomy, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
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  • Caroline Maake,

    1. Institute of Anatomy, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
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  • Greta R. Patzke

    Corresponding author
    1. Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland, Fax: (+) 41 44 635 6802
    • Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland, Fax: (+) 41 44 635 6802.
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Abstract

Polyoxometalate/carboxymethyl chitosan nanocomposites with an average diameter of 130 nm are synthesized and labeled with fluorescein isothiocyanate (FITC) for a combined drug-carrier and cellular-monitoring approach. [Eu(β2-SiW11O39)2]13−/CMC nanospheres as a representative example do not display cytotoxicity for POM concentrations up to 2 mg mL−1. Cellular uptake of fluoresecently labelled {EuSiW11O39}/FITC-CMC nanoparticles is monitored with confocal laser scanning microscopy. Nanoparticle uptake occurs after incubation times of around 1 h and no cyctotoxic effects are observed upon prolonged treatment. The preferential location of the POM/CMC nanocomposites in the perinuclear region is furthermore verified with transmission electron microscopy investigations on unlabeled nanoparticles. Therefore, this approach is a promising dual strategy for the safe cellular transfer and monitoring of bioactive POMs.

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