Advertisement

Allergen Arrays for Antibody Screening and Immune Cell Activation Profiling Generated by Parallel Lipid Dip-Pen Nanolithography

Authors

  • Sylwia Sekula-Neuner,

    Corresponding author
    1. Karlsruher Institut für Technologie (KIT), Institut für Nanotechnologie (INT), Karlsruhe Nano Micro Facility (KNMF), 76021 Karlsruhe Germany
    • Karlsruher Institut für Technologie (KIT), Institut für Nanotechnologie (INT), Karlsruhe Nano Micro Facility (KNMF), 76021 Karlsruhe Germany.
    Search for more papers by this author
  • Jana Maier,

    1. Karlsruher Institut für Technologie, Institut für Toxikologie und Genetik, 76021 Karlsruhe, Germany
    Search for more papers by this author
  • Emmanuel Oppong,

    1. Karlsruher Institut für Technologie, Institut für Toxikologie und Genetik, 76021 Karlsruhe, Germany
    Search for more papers by this author
  • Andrew C. B. Cato,

    1. Karlsruher Institut für Technologie, Institut für Toxikologie und Genetik, 76021 Karlsruhe, Germany
    Search for more papers by this author
  • Michael Hirtz,

    1. Karlsruher Institut für Technologie (KIT), Institut für Nanotechnologie (INT), Karlsruhe Nano Micro Facility (KNMF), 76021 Karlsruhe Germany
    Search for more papers by this author
  • Harald Fuchs

    1. Karlsruher Institut für Technologie (KIT), Institut für Nanotechnologie (INT), Karlsruhe Nano Micro Facility (KNMF), 76021 Karlsruhe Germany
    2. Physikalisches Institut, Universität Münster and Center for Nanotechnology (CeNTech), 48149 Münster, Germany
    Search for more papers by this author

Abstract

Multiple-allergen testing for high throughput and high sensitivity requires the development of miniaturized immunoassays that allow for a large test area and require only a small volume of the test analyte, which is often available only in limited amounts. Developing such miniaturized biochips containing arrays of test allergens needs application of a technique able to deposit molecules at high resolution and speed while preserving its functionality. Lipid dip-pen nanolithography (L-DPN) is an ideal technique to create such biologically active surfaces, and it has already been successfully applied for the direct, nanoscale deposition of functional proteins, as well as for the fabrication of biochemical templates for selective adsorption. The work presented here shows the application of L-DPN for the generation of arrays of the ligand 2,4-dinitrophenyl[1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[6-[(2,4-dinitrophenyl)amino]hexanoyl] (DNP)] onto glass surfaces as a model system for detection of allergen-specific Immunoglobin E (IgE) antibodies and for mast cell activation profiling.

Ancillary