Communication

A Novel Assay for Quantifying the Number of Plasmids Encapsulated by Polymer Nanoparticles

  1. Nupura S. Bhise1,
  2. Ron B. Shmueli1,
  3. Jose Gonzalez1,
  4. Jordan J. Green1,2,*

Article first published online: 5 DEC 2011

DOI: 10.1002/smll.201101718

Issue

Small

Small

Volume 8, Issue 3, pages 367–373, February 6, 2012

Additional Information

How to Cite

Bhise, N. S., Shmueli, R. B., Gonzalez, J. and Green, J. J. (2012), A Novel Assay for Quantifying the Number of Plasmids Encapsulated by Polymer Nanoparticles. Small, 8: 367–373. doi: 10.1002/smll.201101718

Author Information

  1. 1

    Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA

  2. 2

    400 North Broadway, Smith Building Room 5017, Baltimore, MD 21231, USA

*Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.

Publication History

  1. Issue published online: 30 JAN 2012
  2. Article first published online: 5 DEC 2011
  3. Manuscript Revised: 8 OCT 2011
  4. Manuscript Received: 22 AUG 2011
Corrected by:

Corrigendum: A Novel Assay for Quantifying the Number of Plasmids Encapsulated by Polymer Nanoparticles

Vol. 8, Issue 8, 1129, Article first published online: 28 MAR 2012

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Keywords:

  • polymers;
  • nanoparticles;
  • nanoparticle tracking;
  • gene expression;
  • regenerative medicine
Thumbnail image of graphical abstract

Polymeric nanoparticles are promising for gene therapy and stem cell reprogramming using nonviral vectors. A novel assay utilizing nanoparticle tracking analysis is developed to easily quantify the number of plasmids within polymeric nanoparticles while in aqueous solution. Particles effective at co-transfecting primary human fibroblasts are approximately 100 nm in diameter and contain around 100 plasmids per particle.

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