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Keywords:

  • polymers;
  • nanoparticles;
  • nanoparticle tracking;
  • gene expression;
  • regenerative medicine
Thumbnail image of graphical abstract

Polymeric nanoparticles are promising for gene therapy and stem cell reprogramming using nonviral vectors. A novel assay utilizing nanoparticle tracking analysis is developed to easily quantify the number of plasmids within polymeric nanoparticles while in aqueous solution. Particles effective at co-transfecting primary human fibroblasts are approximately 100 nm in diameter and contain around 100 plasmids per particle.