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A Novel Assay for Quantifying the Number of Plasmids Encapsulated by Polymer Nanoparticles

Authors

  • Nupura S. Bhise,

    1. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
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  • Ron B. Shmueli,

    1. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
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  • Jose Gonzalez,

    1. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
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  • Jordan J. Green

    Corresponding author
    1. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    2. 400 North Broadway, Smith Building Room 5017, Baltimore, MD 21231, USA
    • Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.
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Errata

This article is corrected by:

  1. Errata: A Novel Assay for Quantifying the Number of Plasmids Encapsulated by Polymer Nanoparticles Volume 8, Issue 8, 1129, Article first published online: 28 March 2012

Abstract

original image

Polymeric nanoparticles are promising for gene therapy and stem cell reprogramming using nonviral vectors. A novel assay utilizing nanoparticle tracking analysis is developed to easily quantify the number of plasmids within polymeric nanoparticles while in aqueous solution. Particles effective at co-transfecting primary human fibroblasts are approximately 100 nm in diameter and contain around 100 plasmids per particle.

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