Targetable Gold Nanorods for Epithelial Cancer Therapy Guided by Near-IR Absorption Imaging

Authors

  • Jihye Choi,

    1. Department of Chemical and Bimolecular Engineering, Yonsei University, Seoul 120–749, Republic of Korea
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  • Jaemoon Yang,

    1. Department of Radiology, Yonsei University, Seoul 120–752, Republic of Korea
    2. YUHS-KRIBB Medical Convergence Research Institute, Seoul 120–752, Republic of Korea
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  • Doyeon Bang,

    1. Department of Chemical and Bimolecular Engineering, Yonsei University, Seoul 120–749, Republic of Korea
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  • Joseph Park,

    1. Department of Chemical and Bimolecular Engineering, Yonsei University, Seoul 120–749, Republic of Korea
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  • Jin-Suck Suh,

    1. Department of Radiology, Yonsei University, Seoul 120–752, Republic of Korea
    2. YUHS-KRIBB Medical Convergence Research Institute, Seoul 120–752, Republic of Korea
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  • Yong-Min Huh,

    Corresponding author
    1. Department of Radiology, Yonsei University, Seoul 120–752, Republic of Korea
    2. YUHS-KRIBB Medical Convergence Research Institute, Seoul 120–752, Republic of Korea
    • Department of Radiology, Yonsei University, Seoul 120–752, Republic of Korea.
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  • Seungjoo Haam

    Corresponding author
    1. Department of Chemical and Bimolecular Engineering, Yonsei University, Seoul 120–749, Republic of Korea
    2. YUHS-KRIBB Medical Convergence Research Institute, Seoul 120–752, Republic of Korea
    • Department of Chemical and Bimolecular Engineering, Yonsei University, Seoul 120–749, Republic of Korea
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Abstract

Well-designed nanoparticle-mediated, image-guided cancer therapy has attracted interest for increasing the efficacy of cancer treatment. A new class of smart theragnostic nanoprobes employing cetuximab (CET)-conjugated polyethylene glycol (PEG)ylated gold nanorods (CET-PGNRs) is presented; these nanoprobes target epithelial cancer cells using near-infrared light. The cetyltrimethylammonium bromide bilayer on GNRs is replaced with heterobifunctional PEG (COOH-PEG-SH) to serve as a biocompatible stabilizer and to increase specificity. The carboxylated GNRs are further functionalized with CET using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC-NHS) chemistry. To assess the potential of such GNRs, their optical properties, biocompatibility, colloidal stability, in vitro/in vivo binding affinities for cancer cells, absorption imaging, and photothermal therapy effects are investigated. CET-PGNRs exhibit excellent tumor targeting ability and strong potential for simultaneous absorption imaging and photothermal ablation of epithelial cancer cells.

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