Fiber-Optic High-Resolution Genetic Screening Using Gold-Labeled Gene Probes



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A new, ultra-sensitive genetic screening tool based on a fiber-optic (FO) surface plasmon biosensor and gold-labeled gene probes is presented. The assay allows for the detection and location of single-point mutations (SNP) at significantly reduced cost and high speed. The assay is evaluated for SNP detection of 3 different DNA targets, including a GC-rich cancer gene fragment and a long bacterial sequence. The data proves that the accuracy of the FO-SNP test is as good or, in some applications, better than classical high-resolution melting (HRM).