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Keywords:

  • magnetic nanoparticles;
  • magnetofluorescence;
  • fusion proteins;
  • bioconjugation;
  • tumor targeting
Thumbnail image of graphical abstract

A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the site-specific covalent conjugation of targeting peptides to nanoparticles. An scFv mutant selective for HER2 tumor antigen is chosen as the targeting ligand. SNAP-scFv is immobilized on magnetofluorescent nanoparticles and its targeting efficiency against HER2-positive cells is assessed by flow cytometry and immunofluorescence.