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Self-Assembling Peptide-Based Nanoparticles for siRNA Delivery in Primary Cell Lines

Authors

  • Sébastien Deshayes,

    1. Centre de Recherches de Biochimie Macromoléculaire, Department of Molecular Biophysics and Therapeutics, CNRS UMR-5237, UM1-UM2, University of Montpellier, 1919 Route de Mende, 34293 Montpellier, France
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Karidia Konate,

    1. Centre de Recherches de Biochimie Macromoléculaire, Department of Molecular Biophysics and Therapeutics, CNRS UMR-5237, UM1-UM2, University of Montpellier, 1919 Route de Mende, 34293 Montpellier, France
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Anna Rydström,

    1. Centre de Recherches de Biochimie Macromoléculaire, Department of Molecular Biophysics and Therapeutics, CNRS UMR-5237, UM1-UM2, University of Montpellier, 1919 Route de Mende, 34293 Montpellier, France
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  • Laurence Crombez,

    1. Centre de Recherches de Biochimie Macromoléculaire, Department of Molecular Biophysics and Therapeutics, CNRS UMR-5237, UM1-UM2, University of Montpellier, 1919 Route de Mende, 34293 Montpellier, France
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  • Cédric Godefroy,

    1. Centre de Biochimie Structurale, Inserm, U 554, Montpellier, France
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  • Pierre-Emmanuel Milhiet,

    1. Centre de Biochimie Structurale, Inserm, U 554, Montpellier, France
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  • Annick Thomas,

    1. CBMN, Gembloux AgroBiotech, ULG, 2 passage des déportés, 5030 Gembloux, Belgium
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  • Robert Brasseur,

    1. CBMN, Gembloux AgroBiotech, ULG, 2 passage des déportés, 5030 Gembloux, Belgium
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  • Gudrun Aldrian,

    1. Centre de Recherches de Biochimie Macromoléculaire, Department of Molecular Biophysics and Therapeutics, CNRS UMR-5237, UM1-UM2, University of Montpellier, 1919 Route de Mende, 34293 Montpellier, France
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  • Frédéric Heitz,

    1. Centre de Recherches de Biochimie Macromoléculaire, Department of Molecular Biophysics and Therapeutics, CNRS UMR-5237, UM1-UM2, University of Montpellier, 1919 Route de Mende, 34293 Montpellier, France
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  • Maria A. Muñoz-Morris,

    1. Centro Nacional de Investigaciones Metalurgicas-CSIC, Avda Gregorio del Amo 8, 28040 Madrid, Spain
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  • Jean-Marie Devoisselle,

    1. Institut Charles Gerhardt Montpellier, UMR 5253, CNRS-UM2-ENSCM-UM1, Montpellier, France
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  • Gilles Divita

    Corresponding author
    1. Centre de Recherches de Biochimie Macromoléculaire, Department of Molecular Biophysics and Therapeutics, CNRS UMR-5237, UM1-UM2, University of Montpellier, 1919 Route de Mende, 34293 Montpellier, France
    • Centre de Recherches de Biochimie Macromoléculaire, Department of Molecular Biophysics and Therapeutics, CNRS UMR-5237, UM1-UM2, University of Montpellier, 1919 Route de Mende, 34293 Montpellier, France.
    Search for more papers by this author

Abstract

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The secondary amphipathic peptide CADY forms stable positively charged nanoparticles with short interfering RNA (siRNA), which involves both electrostatic and hydrophobic interactions. CADY/siRNA self-assembling nanoparticles adopt a “raspberry”-like architecture cemented together by a matrix of free peptides. These nanoparticles are nontoxic and promote efficient delivery of siRNA in challenging primary cell lines.

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