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Sensitive Detection of Spores Using Volume-Amplified Magnetic Nanobeads

Authors

  • Teresa Zardán Gómez de la Torre,

    1. Department of Engineering Sciences, Division for Nanotechnology and Functional Materials, The Ångström Laboratory, Uppsala University, Box 534, 75121 Uppsala, Sweden
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Rongqin Ke,

    1. Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Rudbeck Laboratory, Uppsala University, 75185 Uppsala, Sweden
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Anja Mezger,

    1. Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Rudbeck Laboratory, Uppsala University, 75185 Uppsala, Sweden
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  • Peter Svedlindh,

    1. Department of Engineering Sciences, Division for Solid State Physics, The Ångström Laboratory, Uppsala University, Box 534, 75121 Uppsala, Sweden
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  • Maria Strømme,

    Corresponding author
    1. Department of Engineering Sciences, Division for Nanotechnology and Functional Materials, The Ångström Laboratory, Uppsala University, Box 534, 75121 Uppsala, Sweden
    • Department of Engineering Sciences, Division for Nanotechnology and Functional Materials, The Ångström Laboratory, Uppsala University, Box 534, 75121 Uppsala, Sweden
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  • Mats Nilsson

    Corresponding author
    1. Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Rudbeck Laboratory, Uppsala University, 75185 Uppsala, Sweden
    • Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Rudbeck Laboratory, Uppsala University, 75185 Uppsala, Sweden.
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Abstract

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A magnetic-nanobead-based, substrate-free method for the sensitive detection of spores in an immunoassay format is presented. The method is shown to detect Bacillus globigii spores, the non-pathogenic simulant of Bacillus anthracis, with a limit-of-detection of 50 spores with a reaction time of 135 min. The study shows the versatility of magnetic nanobeads for detection of biological molecules other than DNA.

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