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Cancer Prognostics by Direct Detection of p53-Antibodies on Gold Surfaces by Impedance Measurements

Authors

  • Elisabet Prats-Alfonso,

    Corresponding author
    1. Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028-Barcelona, Spain
    2. CIBER-BBN, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028, Barcelona, Spain
    3. Department of Organic Chemistry, University of Barcelona, Martí i Franqués 1-11, 08028-Barcelona, Spain
    • Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028-Barcelona, Spain.
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  • Xavier Sisquella,

    1. Nanotechnology Platform, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028, Barcelona, Spain
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  • Nadia Zine,

    1. Université Lyon, Université Claude Bernard-Lyon 1, UMR 5180 institut des Sciences Analytiques, Bat CPE, 43, Bd du 11 novembre 1918, 69622, Villeurbanne Cedex, France
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  • Gemma Gabriel,

    1. Institut de Microelectrònica de Barcelona (IMB-CNM), CSIC, Campus Universitat Autònoma de Barcelona, Barcelona 08193, Spain
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  • Anton Guimerà,

    1. Institut de Microelectrònica de Barcelona (IMB-CNM), CSIC, Campus Universitat Autònoma de Barcelona, Barcelona 08193, Spain
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  • F. Javier del Campo,

    1. Institut de Microelectrònica de Barcelona (IMB-CNM), CSIC, Campus Universitat Autònoma de Barcelona, Barcelona 08193, Spain
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  • Rosa Villa,

    1. Institut de Microelectrònica de Barcelona (IMB-CNM), CSIC, Campus Universitat Autònoma de Barcelona, Barcelona 08193, Spain
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  • Adam H. Eisenberg,

    1. Howard Hughes Medical Institute and Northwestern University, Departments of Chemistry and Biomedical Engineering, 2145 Sheridan Road, Evanston IL 60208
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  • Milan Mrksich,

    1. Howard Hughes Medical Institute and Northwestern University, Departments of Chemistry and Biomedical Engineering, 2145 Sheridan Road, Evanston IL 60208
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  • Abdelhamid Errachid,

    1. Université Lyon, Université Claude Bernard-Lyon 1, UMR 5180 institut des Sciences Analytiques, Bat CPE, 43, Bd du 11 novembre 1918, 69622, Villeurbanne Cedex, France
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  • Jordi Aguiló,

    1. Institut de Microelectrònica de Barcelona (IMB-CNM), CSIC, Campus Universitat Autònoma de Barcelona, Barcelona 08193, Spain
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  • Fernando Albericio

    Corresponding author
    1. Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028-Barcelona, Spain
    2. CIBER-BBN, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028, Barcelona, Spain
    3. Department of Organic Chemistry, University of Barcelona, Martí i Franqués 1-11, 08028-Barcelona, Spain
    • Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, Baldiri Reixac 10, 08028-Barcelona, Spain.
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Abstract

The identification and measurement of biomarkers is critical to a broad range of methods that diagnose and monitor many diseases. Serum auto-antibodies are rapidly becoming interesting targets because of their biological and medical relevance. This paper describes a highly sensitive, label-free approach for the detection of p53-antibodies, a prognostic indicator in ovarian cancer as well as a biomarker in the early stages of other cancers. This approach uses impedance measurements on gold microelectrodes to measure antibody concentrations at the picomolar level in undiluted serum samples. The biosensor shows high selectivity as a result of the optimization of the epitopes responsible for the detection of p53-antibodies and was validated by several techniques including microcontact printing, self-assembled-monolayer desorption ionization (SAMDI) mass spectrometry, and adhesion pull-off force by atomic force microscopy (AFM). This transduction method will lead to fast and accurate diagnostic tools for the early detection of cancer and other diseases.

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