Single-Particle Tracking: Single-Particle Tracking of Hepatitis B Virus-like Vesicle Entry into Cells (Small 9/2011)

Authors

  • Xian Hao,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China
    2. Graduate School of Chinese Academy of Sciences, Beijing 100049, P. R. China
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  • Xin Shang,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China
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  • Jiazhen Wu,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China
    2. Graduate School of Chinese Academy of Sciences, Beijing 100049, P. R. China
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  • Yuping Shan,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China
    2. Graduate School of Chinese Academy of Sciences, Beijing 100049, P. R. China
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  • Mingjun Cai,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China
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  • Junguang Jiang,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China
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  • Zhong Huang,

    Corresponding author
    1. Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025, P. R. China, Tel.:+86-21-54653077; Fax: +86-21-63843571
    • Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025, P. R. China, Tel.:+86-21-54653077; Fax: +86-21-63843571
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  • Zhiyong Tang,

    Corresponding author
    1. National Center for Nanoscience and Technology, Beijing 100190, P. R. China
    • National Center for Nanoscience and Technology, Beijing 100190, P. R. China.
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  • Hongda Wang

    Corresponding author
    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China
    • State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China
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  • Dedicated to Professor Shaojun Dong on the occasion of her 80th birthday

Abstract

original image

The cover image shows the visualization of the entry of a Hepatitis B virus-like vesicle (HBsAg) into a cell. HBsAg (shown as red particles) is used as a model to study the infection mechanisms and dynamics of the single-enveloped virus in living cells by real-time fluorescence microscopy. HBsAg is found to enter cells via a caveolin-mediated endocytic pathway (caveolin: yellow). By tracking individual HBsAg particles in living cells, the anomalously actin-dependent but not microtubule-dependent motility of internalized HBsAg particles is revealed (actin: green). The motility of HBsAg particles in living cells was also analyzed quantitatively. The results may potentially settle the long-term debate of whether hepatitis B viruses directly break the plasma membrane barrier or rely on endocytosis to infect the cell. For more information, please read the Full Paper “Single-Particle Tracking of Hepatitis B Virus-like Vesicle Entry into Cells” by Z. Huang, Z. Tang, H. Wang, and co-workers, beginning on page 1212.

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