Superparamagnetic Nanoparticles: N-Alkyl-PEI-Functionalized Iron Oxide Nanoclusters for Efficient siRNA Delivery (Small 19/2011)

Authors

  • Gang Liu,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA
    2. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China
    3. Sichuan Key Laboratory of Medical Imaging, Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, China
    Current affiliation:
    1. These authors contributed equally in this work.
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  • Jin Xie,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA
    Current affiliation:
    1. These authors contributed equally in this work.
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  • Fan Zhang,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA
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  • Zhiyong Wang,

    1. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China
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  • Kui Luo,

    1. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China
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  • Lei Zhu,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA
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  • Qimeng Quan,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA
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  • Gang Niu,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA
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  • Seulki Lee,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA
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  • Hua Ai,

    Corresponding author
    1. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China
    2. Department of Radiology, West China Hospital, Sichuan University, Chengdu, 610041, China
    • National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China
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  • Xiaoyuan Chen

    Corresponding author
    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA
    • Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA.
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Abstract

original image

The cover illustrates a multifunctional gene delivery system interacting with cancer cells. Small interfering RNA (siRNA) is an emerging class of therapeutics, which works by regulating the expression of a specifi c gene involved in disease progression. Effective transport of siRNA with minimal side effects remains a challenge. In this study, a nonviral nanoparticle gene carrier was developed which contains a core of clustered superparamagnetic iron oxide nanoparticles and a shell of alkylated polyethyleneimine. This composite effectively binds siRNA with good protection, as demonstrated by an enzymatic degradation assay. Signifi cant down-regulation of luciferase by loaded nanocarriers was observed both in vitro and in vivo. In addition, transfected cells showed signifi cant MR imaging contrast compared to nontransfected ones. For more information, please read the Full paper “N-Alkyl-PEI-Functionalized Iron Oxide Nanoclusters for Effi cient siRNA Delivery” by H. Ai, X. Chen, and co-workers beginning on page 2742.

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