Control of Cell Adhesion and Neurite Outgrowth by Patterned Gold Nanoparticles with Tunable Attractive or Repulsive Surface Properties

Authors

  • Sandra Gilles,

    1. Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52428 Jülich, Germany
    2. JARA—Fundamentals of Future Information Technology, Germany
    3. Institute of Inorganic Chemistry, RWTH Aachen University, 52074 Aachen, Germany
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  • Silke Winter,

    1. Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52428 Jülich, Germany
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  • Kristin E. Michael,

    1. Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52428 Jülich, Germany
    Current affiliation:
    1. Published post-humously, deceased January 2011
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  • Simone H. Meffert,

    1. Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52428 Jülich, Germany
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  • Pinggui Li,

    1. Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52428 Jülich, Germany
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  • Kyrylo Greben,

    1. Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52428 Jülich, Germany
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  • Ulrich Simon,

    1. JARA—Fundamentals of Future Information Technology, Germany
    2. Institute of Inorganic Chemistry, RWTH Aachen University, 52074 Aachen, Germany
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  • Andreas Offenhäusser,

    1. Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52428 Jülich, Germany
    2. JARA—Fundamentals of Future Information Technology, Germany
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  • Dirk Mayer

    Corresponding author
    1. Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52428 Jülich, Germany
    2. JARA—Fundamentals of Future Information Technology, Germany
    • Peter Grünberg Institute (PGI-8), Forschungszentrum Jülich GmbH, 52428 Jülich, Germany.
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Abstract

Guiding of neuronal cells on surfaces is required for the investigation of fundamental aspects of neurobiology, for tissue engineering, and for numerous bioelectronic applications. A modular method to establish nanostructured chemical templates for local deposition of gold nanoparticles is presented. A process comprising nanoimprint lithography, silanization, lift-off, and gold nanoparticle immobilization is used to fabricate the particle patterns. The chemical composition of the surface can be modified by in situ adsorption of cell-binding ligands to locally addressed particles. The versatility of this approach is demonstrated by inverting the binding affinity between rat cortical neurons and nanopatterned surfaces via wet-chemical means and thereby reversing the pattern of guided neurons.

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