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A Supramolecular Approach to Enzyme Immobilization in Micro-Channels

Authors

  • Arántzazu González-Campo,

    1. Molecular Nanofabrication Group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE, Netherlands
    Current affiliation:
    1. A.G.-C. and B.E. contributed equally to this work.
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  • Bilge Eker,

    1. Mesoscale Chemical Systems Group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE, Netherlands
    Current affiliation:
    1. A.G.-C. and B.E. contributed equally to this work.
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  • Han J. G. E. Gardeniers,

    1. Mesoscale Chemical Systems Group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE, Netherlands
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  • Jurriaan Huskens,

    Corresponding author
    1. Molecular Nanofabrication Group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE, Netherlands
    • Molecular Nanofabrication Group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE, Netherlands.
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  • Pascal Jonkheijm

    Corresponding author
    1. Molecular Nanofabrication Group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE, Netherlands
    • Molecular Nanofabrication Group, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE, Netherlands.
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Abstract

A supramolecular assembly scheme is developed to enable the facile in-situ immobilization of enzymes in a microfluidic channel system. A combination of orthogonal supramolecular interactions of host (β-cyclodextrin)–guest (adamantane) and biotin–Streptavidin (SAv) interactions are employed to generate reusable homogeneous enzyme layers in microchannels. The structural integrity and catalytic activity of the immobilized enzyme calf-intestine alkaline phosphatase (AlkPh) is demonstrated. From the kinetic analysis of a dephosphorylation reaction, the specificity constant kcat/KM for immobilized alkaline phosphatase in the channels is on the order of 105 M−1s−1 and comparable to known literature values in other environments. These observations are ascribed to the good access of the substrate to favorably oriented enzymes across the microchannel. Therefore, this study demonstrates the great potential for adopting a supramolecular assembly scheme to immobilize enzymes in microfluidic devices.

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