Fabrication of Novel Hierarchical Structured Fe3O4@LnPO4 (Ln=Eu, Tb, Er) Multifunctional Microspheres for Capturing and Labeling Phosphopeptides

Authors

  • Zhi-Gang Wang,

    1. State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, PR China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, PR China
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  • Gong Cheng,

    1. State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, PR China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, PR China
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  • Yan-Lin Liu,

    1. State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, PR China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, PR China
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  • Ji-Lin Zhang,

    Corresponding author
    1. State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, PR China
    • State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, PR China.
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  • De-Hui Sun,

    1. Changchun Institute of Technology, Changchun 130012, PR China
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  • Jia-Zuan Ni

    1. State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, PR China
    2. Key Laboratory of Marine Bioresources and Ecology, College of Life Science, Shenzhen University, Shenzhen 518060, PR China
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Abstract

Novel core–shell structured Fe3O4@LnPO4 (Ln=Eu, Tb, Er) multifunctional microspheres with a magnetic Fe3O4 core and a LnPO4 shell covered with spikes are synthesized for the first time through the combination of a homogeneous precipitation approach and an ion-exchange process. Their potential for selective capture, rapid separation, and easy mass spectrometry (MS) labeling of the phosphopeptides from complex proteolytic digests are evaluated. These affinity microspheres can improve the specificity for capture of the phosphopeptides, realize fast magnetic separation, enhance the MS detection signals, and directly identify phosphopeptides through 80 Da mass loss in the mass spectra. The synthesis strategy could become a general and effective technique for similar core–shell hierarchical structures.

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